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dc.contributor.authorBesuschio, Susana A
dc.contributor.authorPicado, Albert
dc.contributor.authorMuñoz-Calderón, Arturo
dc.contributor.authorWehrendt, Diana P
dc.contributor.authorFernández, Marisa
dc.contributor.authorBenatar, Alejandro
dc.contributor.authorDiaz-Bello, Zoraida
dc.contributor.authorIrurtia, Cecilia
dc.contributor.authorCruz, Israel 
dc.contributor.authorNdung'u, Joseph M
dc.contributor.authorCafferata, María L
dc.contributor.authorMontenegro, Graciela
dc.contributor.authorSosa Estani, Sergio
dc.contributor.authorLucero, Raúl H.
dc.contributor.authorAlarcón de Noya, Belkisyole
dc.contributor.authorLonghi, Silvia A
dc.contributor.authorSchijman, Alejandro G
dc.date.accessioned2020-09-22T10:05:18Z
dc.date.available2020-09-22T10:05:18Z
dc.date.issued2020
dc.identifier.citationPLoS Negl Trop Dis . 2020 Aug 14;14(8):e0008402.es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/11064
dc.description.abstractA Trypanosoma cruzi Loopamp kit was recently developed as a ready-to-use diagnostic method requiring minimal laboratory facilities. We evaluated its diagnostic accuracy for detection of acute Chagas disease (CD) in different epidemiological and clinical scenarios. In this retrospective study, a convenience series of clinical samples (venous blood treated with EDTA or different stabilizer agents, heel-prick blood in filter paper or cerebrospinal fluid samples (CSF)) from 30 infants born to seropositive mothers (13 with congenital CD and 17 noninfected), four recipients of organs from CD donors, six orally-infected cases after consumption of contaminated guava juice and six CD patients coinfected with HIV at risk of CD reactivation (N = 46 patients, 46 blood samples and 1 CSF sample) were tested by T. cruzi Loopamp kit (Tc LAMP) and standardized quantitative real-time PCR (qPCR). T. cruzi Loopamp accuracy was estimated using the case definition in the different groups as a reference. Cohen's kappa coefficient (κ) was applied to measure the agreement between Tc LAMP (index test) and qPCR (reference test). Sensitivity and specificity of T. cruzi Loopamp kit in blood samples from the pooled clinical groups was 93% (95% CI: 77-99) and 100% (95% CI: 80-100) respectively. The agreement between Tc LAMP and qPCR was almost perfect (κ = 0.92, 95% CI: 0.62-1.00). The T. cruzi Loopamp kit was sensitive and specific for detection of T. cruzi infection. It was carried out from DNA extracted from peripheral blood samples (via frozen EDTA blood, guanidine hydrochloride-EDTA blood, DNAgard blood and dried blood spots), as well as in CSF specimens infected with TcI or TcII/V/VI parasite populations. The T. cruzi Loopamp kit appears potentially useful for rapid detection of T. cruzi infection in congenital, acute and CD reactivation due to HIV infection.es_ES
dc.description.sponsorshipFunding for this study was received from the German Federal Ministry of Education and Research (BMBF) through the KfW Entwicklungs bank. FIND is grateful to its donors, public and private, who have helped to support the development of innovative new diagnostics for diseases of poverty. A list of FIND donors can be found at: https://www.finddx.org/partners-donors/. The study was also partially funded by PICT 2015-0074 from the Argentinean Ministry of Science Technology and Innovation and ERANet -LAC HD 328 from Network of the European Union (EU), Latin America and the Caribbean Countries (LAC) on Joint Innovation and Research Activities to AGS. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Science (PLOS) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshChagas Disease es_ES
dc.subject.meshCoinfection es_ES
dc.subject.meshDNA, Protozoan es_ES
dc.subject.meshFemale es_ES
dc.subject.meshHIV Infections es_ES
dc.subject.meshHumans es_ES
dc.subject.meshInfant es_ES
dc.subject.meshInfant, Newborn es_ES
dc.subject.meshMale es_ES
dc.subject.meshNucleic Acid Amplification Techniques es_ES
dc.subject.meshReal-Time Polymerase Chain Reaction es_ES
dc.subject.meshRetrospective Studies es_ES
dc.subject.meshSensitivity and Specificity es_ES
dc.subject.meshTransplant Recipients es_ES
dc.subject.meshTrypanosoma cruzi es_ES
dc.titleTrypanosoma cruzi loop-mediated isothermal amplification (Trypanosoma cruzi Loopamp) kit for detection of congenital, acute and Chagas disease reactivation.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID32797041es_ES
dc.format.volume14es_ES
dc.format.number8es_ES
dc.format.pagee0008402es_ES
dc.identifier.doi10.1371/journal.pntd.0008402es_ES
dc.contributor.funderKfW Development Bank 
dc.contributor.funderMinisterio de Ciencia, Tecnología e Innovación (Argentina) 
dc.contributor.funderLatin America and the Caribbean Countries (LAC)
dc.contributor.funderUnión Europea 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1935-2735es_ES
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pntd.0008402es_ES
dc.identifier.journalPLoS neglected tropical diseaseses_ES
dc.repisalud.centroISCIII::Escuela Nacional de Sanidades_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PICT 2015-0074es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/ERANet -LAC HD 328es_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional