Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/10888
Título
Hepatitis C Virus Influences HIV-1 Viral Splicing in Coinfected Patients
Autor(es)
Martinez-Roman, Paula ISCIII | Lopez-Huertas, Maria Rosa ISCIII | Crespo-Bermejo, Celia ISCIII | Cortegano, Isabel ISCIII | Valle-Millares, Daniel ISCIII | Gaspar, Maria Luisa ISCIII | Martín-Carbonero, Luz | Domínguez-Domínguez, Lourdes | Ryan, Pablo | de los Santos, Ignacio | de la Fuente-Moral, Sara | Fernandez-Rodriguez, Amanda ISCIII | Coiras, Mayte ISCIII | Briz, Veronica ISCIII
Fecha de publicación
2020
Cita
J Clin Med. 2020;9(7):E2091
Idioma
Inglés
Tipo de documento
journal article
Resumen
Coinfection with hepatitis C virus (HCV) influences HIV reservoir size. However, it is unknown whether this coinfection also induces a higher provirus transcription. Viral transcription is promoted by synergy between cellular factors such as NF-κB and the viral regulator Tat. The impact of HCV coinfection on HIV provirus transcription was analyzed in resting (r)CD4 T+ cells (CD3+CD4+CD25-CD69-HLADR-) and rCD4 T cells-depleted PBMCs (rCD4 T- PBMCs) from a multicenter cross-sectional study of 115 cART-treated HIV patients: 42 HIV+/HCV+ coinfected individuals (HIV+/HCV+), 34 HIV+ patients with HCV spontaneous clearance (HIV+/HCV−) and 39 HIV patients (HIV+). Viral transcription was assessed in total RNA through the quantification of unspliced, single spliced, and multiple spliced viral mRNAs by qPCR. Linear correlations between viral reservoir size and viral splicing were determined. A 3-fold increase of multiple spliced transcripts in rCD4 T+ cells of HIV+/HCV+ patients was found compared to HIV+ individuals (p < 0.05). As Tat is synthesized by multiple splicing, the levels of Tat were also quantified in these patients. Significant differences in single and multiple spliced transcripts were also observed in rCD4 T- PBMCs. Levels of multiple spliced mRNAs were increased in rCD4 T+ cells isolated from HIV+/HCV+ subjects, which could indicate a higher Tat activity in these cells despite their resting state.
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Aparece en las colecciones
- Investigación > IIS > IRYCIS - Instituto Ramón y Cajal de Investigación Sanitaria (Madrid)
- Investigación > IIS > IdiPAZ - Instituto de Investigación Sanitaria Hospital La Paz (Madrid)
- Investigación > IIS > i+12 - Instituto de Investigación Hospital 12 de Octubre (Madrid)
- Investigación > ISCIII > Centro Nacional de Microbiología (CNM) > ISCIII - Artículos