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dc.contributor.authorRafiei, Abdollah
dc.contributor.authorBaghlaninezhad, Raheleh
dc.contributor.authorPanabad, Esmat
dc.contributor.authorBeiromvand, Molouk
dc.contributor.authorKöster, Pamela Carolina 
dc.contributor.authorBailo-Barroso, Begoña 
dc.contributor.authorHernandez-De-Mingo, Marta 
dc.contributor.authorCarmena, David 
dc.date.accessioned2020-06-18T07:06:40Z
dc.date.available2020-06-18T07:06:40Z
dc.date.issued2020
dc.identifier.citationPLoS One . 2020 Feb 6;15(2):e0228317es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/10486
dc.description.abstractGiardia duodenalis is one of the main enteric pathogens associated with diarrheal disease. In developing countries, giardiasis is a major public health concern, particularly in children under five years of age. This study aimed to evaluate the occurrence and genetic diversity of G. duodenalis causing human infections in Shushtar County, Southwestern Iran. Individual faecal specimens were collected from 1,163 individuals (male/female ratio: 0.9; age range 2-75 years) with (n = 258) and without (n = 905) gastrointestinal symptoms living in rural and urban settings during the period 2017-2018. Conventional (sucrose flotation and microscopy) methods were used for the initial detection of G. duodenalis cysts in faecal specimens. Microscopy-positive samples were confirmed by PCR amplification and sequencing of the small subunit rRNA (ssu rRNA) gene of the parasite. A multilocus genotyping (MLG) scheme targeting the triose phosphate isomerase (tpi), the glutamate dehydrogenase (gdh), and the beta-giardin (bg) genes was used for genotyping purposes. Giardia duodenalis cysts were detected in 7.7% (90/1,163) of samples by microscopy, of which 82 were confirmed by ssu-PCR. Successful amplification and sequencing results were obtained for 23.2% (19/82), 9.8% (8/82), and 8.5% (7/82) of the confirmed samples at the tpi, gdh, and bg loci, respectively. MLG data for the three loci were available for two samples only. Out of the 24 samples genotyped at any loci, 50% (12/24) were identified as assemblage A and the remaining half as assemblage B. Overall, AII was the most prevalent sub-assemblage detected (41.7%, 10/24), followed by BIII (25.0%, 6/24), discordant BIII/BIV (5/24) or AII/AIII (2/24) sequences, and BIV (1/24). No significant correlation was demonstrated between a given assemblage/sub-assemblage and the occurrence of clinical symptoms. No genotypes adapted to animal hosts other than humans (e.g. assemblages C-F) were found circulating in the investigated human population, suggesting that transmission of human giardiasis in this Iranian region is primarily of anthroponotic nature. Further molecular-based studies are needed to confirm and expand these results, and to ascertain the presence and public health relevance of the parasite in environmental (e.g. drinking water) samples.es_ES
dc.description.sponsorshipThis study was funded by the Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz (Iran) under grant OG/9731 to MB. Additional funding was obtained from the Health Institute Carlos III (ISCIII), Ministry of Economy and Competitiveness (Spain), under project PI16CIII/00024 to DC. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Science (PLOS) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshMultilocus Sequence Typing es_ES
dc.subject.meshAdolescent es_ES
dc.subject.meshAdult es_ES
dc.subject.meshAged es_ES
dc.subject.meshChild es_ES
dc.subject.meshChild, Preschool es_ES
dc.subject.meshCytoskeletal Proteins es_ES
dc.subject.meshFemale es_ES
dc.subject.meshGenotype es_ES
dc.subject.meshGiardia lamblia es_ES
dc.subject.meshGiardiasis es_ES
dc.subject.meshGlutamate Dehydrogenase es_ES
dc.subject.meshHumans es_ES
dc.subject.meshIran es_ES
dc.subject.meshMale es_ES
dc.subject.meshMiddle Aged es_ES
dc.subject.meshPhylogeny es_ES
dc.subject.meshProtozoan Proteins es_ES
dc.subject.meshTriose-Phosphate Isomerase es_ES
dc.subject.meshYoung Adult es_ES
dc.titleMultilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID32027684es_ES
dc.format.volume15es_ES
dc.format.number2es_ES
dc.format.pagee0228317es_ES
dc.identifier.doi10.1371/journal.pone.0228317es_ES
dc.contributor.funderInstituto de Salud Carlos III 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1932-6203es_ES
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0228317es_ES
dc.identifier.journalPloS onees_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/OG/9731es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/PI16CIII/00024es_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
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