dc.contributor.author | Bedoya, Luis M | |
dc.contributor.author | Beltran, Manuela | |
dc.contributor.author | Obregon-Calderon, Patricia | |
dc.contributor.author | García-Pérez, Javier | |
dc.contributor.author | de la Torre-Tarazona, Humberto Erick | |
dc.contributor.author | Gonzalez-Fernandez, Nuria | |
dc.contributor.author | Perez-Olmeda, Mayte | |
dc.contributor.author | Auñón, David | |
dc.contributor.author | Capa, Laura | |
dc.contributor.author | Gómez-Acebo, Eduardo | |
dc.contributor.author | Alcamí, José | |
dc.date.accessioned | 2020-06-02T07:31:55Z | |
dc.date.available | 2020-06-02T07:31:55Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | AIDS. 2016 Nov 28;30(18):2767-2776. | es_ES |
dc.identifier.issn | 0269-9370 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/10247 | |
dc.description.abstract | OBJECTIVE: To investigate the toxicity and activity against HIV of 5-hydroxytyrosol as a potential microbicide. DESIGN: The anti-HIV-1 activity of 5-hydroxytyrosol, a polyphenolic compound, was tested against wild-type HIV-1 and viral clones resistant to nucleoside reverse transcriptase inhibitors (NRTIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs), protease inhibitors and integrase inhibitors. In addition to its activity against founder viruses, different viral subtypes and potential synergy with tenofovir disoproxil fumarate, lamivudine and emtricitabine was also tested. 5-Hydroxytyrosol toxicity was evaluated in vivo in rabbit vaginal mucosa. METHODS: We have cloned pol gene from drug-resistant HIV-1 isolated from infected patients and env gene from Fiebeg III/IV patients or A, C, D, E, F and G subtypes in the NL4.3-Ren backbone. 5-Hydroxytyrosol anti-HIV-1 activity was evaluated in infections of MT-2, U87-CCR5 or peripheral blood mononuclear cells preactivated with phytohemagglutinin + interleukin-2 with viruses obtained through 293T transfections. Inhibitory concentration 50% and cytotoxic concentration 50% were calculated. Synergy was analysed according to Chou and Talalay method. In-vivo toxicity was evaluated for 14 days in rabbit vaginal mucosa. RESULTS: 5-Hydroxytyrosol inhibited HIV-1 infections of recombinant or wild-type viruses in all the target cells tested. Moreover, 5-hydroxytyrosol showed similar inhibitory concentration 50% values for infections with NRTIs, NNRTIs, protease inhibitors and INIs resistant viruses; founder viruses and all the subtypes tested. Combination of 5-hydroxytyrosol with tenofovir was found to be synergistic, whereas it was additive with lamivudine and emtricitabine. In-vivo toxicity of 5-hydroxytyrosol was very low even at the highest tested doses. CONCLUSION: 5-Hydroxytyrosol displayed a broad anti-HIV-1 activity in different cells systems in the absent of in-vivo toxicity, therefore supporting its candidacy as a potential new class of microbicides. | es_ES |
dc.description.sponsorship | AIM-HIV Network of Excellence of the EU, grant number HEALTH-F3-2012-305938. CHAARM European Community's Seventh Framework programme (FP7/2007-2013) under grant agreement no. 242135. Instituto de Salud Carlos III (Intrasalud PI12/0056). The following reagents were obtained through the NIH AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH: tenofovir, lamivudine, emtricitabine, nevirapine and raltegravir. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Lippincott Williams & Wilkins (LWW) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Anti-Infective Agents | es_ES |
dc.subject.mesh | Cells, Cultured | es_ES |
dc.subject.mesh | Drug Synergism | es_ES |
dc.subject.mesh | Female | es_ES |
dc.subject.mesh | HIV-1 | es_ES |
dc.subject.mesh | Human Activities | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Leukocytes, Mononuclear | es_ES |
dc.subject.mesh | Microbial Sensitivity Tests | es_ES |
dc.subject.mesh | Phenylethyl Alcohol | es_ES |
dc.subject.mesh | Rabbits | es_ES |
dc.title | Hydroxytyrosol: a new class of microbicide displaying broad anti-HIV-1 activity | es_ES |
dc.type | research article | es_ES |
dc.rights.license | Atribución-NoComercial-SinObraDerivada 4.0 Internacional | * |
dc.identifier.pubmedID | 27677167 | es_ES |
dc.format.volume | 30 | es_ES |
dc.format.number | 18 | es_ES |
dc.format.page | 2767-2776 | es_ES |
dc.identifier.doi | 10.1097/QAD.0000000000001283 | es_ES |
dc.contributor.funder | Unión Europea. Comisión Europea. 7 Programa Marco | |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1473-5571 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1097/QAD.0000000000001283 | es_ES |
dc.identifier.journal | AIDS (London, England) | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/HEALTH-F3-2012-305938 | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/FP7/2007-2013 | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/242135 | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/PI12/0056 | es_ES |
dc.rights.accessRights | open access | es_ES |