Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/10149
Postnatal and adult immunoglobulin repertoires of innate-like CD19(+)CD45R(lo) B Cells
J Innate Immun. 2014;6(4):499-514.
The diversity in antibody repertoire relies on different B cell populations working efficiently to fulfil distinct specific functions. We recently described an innate-like CD19(+)CD45R(-/lo) (19(+)45R(lo)) cell population in postnatal unstimulated adult mice, a heterogeneous population containing cells expressing immunoglobulin M (IgM) and others behaving as differentiated mature B lymphocytes (intracytoplasmic IgG1, AID(+), Blimp-1(+)RAG2(-)). In the present study, we characterized the Ig repertoire expressed by splenic 19(+)45R(lo) cells, assuming that they would bear a restricted repertoire biased for germline rearrangements and low mutation rates similar to other innate-like cells. Sequences from 19(+)45R(lo) cells displayed a variety of V, D and J regions, and the analysis of the CDR-H3 region revealed an intermediate overall CDR-H3 length and moderate hydrophobicity. Both IgM and switched sequences of PD15 19(+)45R(lo) cells had shorter CDR-H3 region and fewer non-template N nucleotides than adult sequences, as expected for profiles that correspond to an immature phenotype. Regarding the mutation rate in the VH regions, IgG1 sequences already carried a high rate of replacement mutations at PD15, which increased further in the sequences obtained from adult mice. Moreover, statistical models suggest that a proportion of the switched sequences in adult 19(+)45R(lo) cells had experienced antigen selection, unlike other innate-like B cell compartments.
Animals | Animals, Newborn | Antibody Diversity | Antigens, CD19 | B-Lymphocyte Subsets | B-Lymphocytes | Cell Differentiation | Cells, Cultured | Immune System | Immunity, Innate | Immunoglobulin Class Switching | Immunoglobulins | Immunologic Memory | Leukocyte Common Antigens | Mice | Mice, Inbred BALB C | V(D)J Recombination
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