Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/8495
Título
Clasp2 ensures mitotic fidelity and prevents differentiation of epidermal keratinocytes
Autor(es)
Shahbazi, Marta N | Peña-Jiménez, Daniel CNIO | Antonucci, Francesca | Drosten, Matthias CNIO | Perez-Moreno, Mirna Alicia CNIO
Fecha de publicación
2017-02-15
Cita
J Cell Sci. 2017;130(4):683-688
Idioma
Inglés
Tipo de documento
journal article
Resumen
Epidermal homeostasis is tightly controlled by a balancing act of self-renewal or terminal differentiation of proliferating basal keratinocytes. An increase in DNA content as a consequence of a mitotic block is a recognized mechanism underlying keratinocyte differentiation, but the molecular mechanisms involved in this process are not yet fully understood. Using cultured primary keratinocytes, here we report that the expression of the mammalian microtubule and kinetochore-associated protein Clasp2 is intimately associated with the basal proliferative makeup of keratinocytes, and its deficiency leads to premature differentiation. Clasp2-deficient keratinocytes exhibit increased centrosomal numbers and numerous mitotic alterations, including multipolar spindles and chromosomal misalignments that overall result in mitotic stress and a high DNA content. Such mitotic block prompts premature keratinocyte differentiation in a p53-dependent manner in the absence of cell death. Our findings reveal a new role for Clasp2 in governing keratinocyte undifferentiated features and highlight the presence of surveillance mechanisms that prevent cell cycle entry in cells that have alterations in the DNA content.
Palabras clave
MESH
Animals | Cell Line, Transformed | Cells, Cultured | DNA Damage | Gene Knockdown Techniques | Humans | Keratinocytes | Mice, Inbred C57BL | Microtubule-Associated Proteins | Tumor Suppressor Protein p53 | Cell Differentiation | Epidermal Cells | Mitosis
Versión en línea
DOI
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