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dc.contributor.authorPimenta-Lopes, Carolina 
dc.contributor.authorSuay-Corredera, Carmen 
dc.contributor.authorVelazquez-Carreras, Diana 
dc.contributor.authorSanchez-Ortiz, David 
dc.contributor.authorAlegre-Cebollada, Jorge 
dc.date.accessioned2019-08-21T07:17:05Z
dc.date.available2019-08-21T07:17:05Z
dc.date.issued2019-08
dc.identifier.citationComms Phys. 2019; 2(1):91es_ES
dc.identifier.issn2399-3650es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/8307
dc.description.abstractForce-spectroscopy by atomic force microscopy (AFM) is the technique of choice to measure mechanical properties of molecules, cells, tissues and materials at the nano and micro scales. However, unavoidable calibration errors of AFM probes make it cumbersome to quantify modulation of mechanics. Here, we show that concurrent AFM force measurements enable relative mechanical characterization with an accuracy that is independent of calibration uncertainty, even when averaging data from multiple, independent experiments. Compared to traditional AFM, we estimate that concurrent strategies can measure differences in protein mechanical unfolding forces with a 6-fold improvement in accuracy or a 30-fold increase in throughput. Prompted by our results, we demonstrate widely applicable orthogonal fingerprinting strategies for concurrent single-molecule nanomechanical profiling of proteins.es_ES
dc.description.sponsorshipJ.A.-C. acknowledges funding from the Ministerio de Ciencia, Innovación y Universidades (MCNU) through grants BIO2014-54768-P, BIO2017-83640-P (AEI/FEDER, UE), and RYC-2014-16604, the European Research Area Network on Cardiovascular Diseases (ERA-CVD/ISCIII, MINOTAUR, AC16/00045), the Comunidad de Madrid (P2018/NMT-4443), and the CNIC-Severo Ochoa intramural grant program (03-2016 IGP). The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), MCNU and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV-2015-0505). C.P.-L. was a recipient of CNIC Master Fellowship. C.S.-C. is the recipient of an FPI predoctoral fellowship BES-2016-076638. We thank Natalia Vicente for excellent technical support (through grant PEJ16/MED/TL-1593 from Comunidad de Madrid).es_ES
dc.language.isoenges_ES
dc.publisherSpringer es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleConcurrent atomic force spectroscopyes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.format.volume2es_ES
dc.format.number1es_ES
dc.format.page91es_ES
dc.identifier.doi10.1038/s42005-019-0192-yes_ES
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España) 
dc.contributor.funderComunidad de Madrid (España) 
dc.contributor.funderCentro Nacional de Investigaciones Cardiovasculares Carlos III (España) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderFundación ProCNIC 
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1038/s42005-019-0192-yes_ES
dc.identifier.journalCommunications Physicses_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Mecánica molecular del sistema cardiovasculares_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BIO2014-54768-Pes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/BIO2017-83640-Pes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RYC-2014-16604es_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional