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dc.contributor.author | Molina-Sanchez, Pedro | |
dc.contributor.author | Andres, Vicente | |
dc.date.accessioned | 2019-07-19T06:28:59Z | |
dc.date.available | 2019-07-19T06:28:59Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Methods Mol Biol. 2015; 1339:111-7 | es_ES |
dc.identifier.isbn | 978-1-4939-2928-3 | es_ES |
dc.identifier.issn | 1064-3745 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/7950 | |
dc.description.abstract | Endothelial cells (ECs) are key blood-vessel-wall components that play critical roles in the regulation of many physiological processes, including angiogenesis, coagulation, and vascular tone control, and in pathological events such as vessel inflammation and leukocyte infiltration. EC dysfunction is one of the first events associated with the development of atherosclerosis and is sustained throughout progression of the disease. The study of ECs in vitro has become an invaluable tool for investigating these vascular processes at the molecular level, and is widely used in the search for therapeutic targets and strategies. This chapter describes a protocol for the isolation and culture of primary mouse aortic ECs based on antibody-mediated EC selection. | es_ES |
dc.description.sponsorship | Work in V.A.’s laboratory is supported by grants from the Spanish Ministry for Economy and Competitiveness (MINECO) (SAF2013-46663-R), the Fondo Europeo de Desarrollo Regional (FEDER), the Instituto de Salud Carlos III (RD12/0042/0028), the Progeria Research Foundation (Innovator Award 2012, Established Investigator Award 2014), and the European Union (Liphos, Grant Agreement 317916). P.M-S. is supported by an FPU predoctoral fellowship from MINECO. The Centro Nacional de Investigaciones Cardiovasculares (CNIC) is supported by the MINECO and the Pro-CNIC Foundation. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer | es_ES |
dc.relation.ispartofseries | Methods in Molelcular Biology | es_ES |
dc.type.hasVersion | AM | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject | Antibody-mediated selection | es_ES |
dc.subject | Atherosclerosis | es_ES |
dc.subject | Culture | es_ES |
dc.subject | Endothelial cells | es_ES |
dc.subject | Isolation | es_ES |
dc.subject | Mouse aorta | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Aorta | es_ES |
dc.subject.mesh | Aortic Diseases | es_ES |
dc.subject.mesh | Atherosclerosis | es_ES |
dc.subject.mesh | Cells, Cultured | es_ES |
dc.subject.mesh | Endothelial Cells | es_ES |
dc.subject.mesh | Immunomagnetic Separation | es_ES |
dc.subject.mesh | Mice | es_ES |
dc.subject.mesh | Primary Cell Culture | es_ES |
dc.title | Isolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodies | es_ES |
dc.type | book part | es_ES |
dc.rights.license | Atribución-NoComercial-CompartirIgual 4.0 Internacional | * |
dc.identifier.pubmedID | 26445784 | |
dc.format.volume | 1339 | |
dc.format.page | 117 | |
dc.identifier.doi | 10.1007/978-1-4939-2929-0_7 | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Progeria Research Foundation | |
dc.contributor.funder | Unión Europea. Comisión Europea | |
dc.contributor.funder | Fundación ProCNIC | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1940-6029 | |
dc.relation.ispartofseriesnumber | 1339 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1007/978-1-4939-2929-0_7 | es_ES |
dc.identifier.journal | Methods in molecular biology (Clifton, N.J.) | |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Fisiopatología Cardiovascular Molecular y Genética | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2013-46663-R | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RD12/0042/0028 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/317916/EU | es_ES |
dc.rights.accessRights | open access | es_ES |