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dc.contributor.authorMolina-Sanchez, Pedro 
dc.contributor.authorAndres, Vicente 
dc.date.accessioned2019-07-19T06:28:59Z
dc.date.available2019-07-19T06:28:59Z
dc.date.issued2015
dc.identifier.citationMethods Mol Biol. 2015; 1339:111-7es_ES
dc.identifier.isbn978-1-4939-2928-3es_ES
dc.identifier.issn1064-3745
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7950
dc.description.abstractEndothelial cells (ECs) are key blood-vessel-wall components that play critical roles in the regulation of many physiological processes, including angiogenesis, coagulation, and vascular tone control, and in pathological events such as vessel inflammation and leukocyte infiltration. EC dysfunction is one of the first events associated with the development of atherosclerosis and is sustained throughout progression of the disease. The study of ECs in vitro has become an invaluable tool for investigating these vascular processes at the molecular level, and is widely used in the search for therapeutic targets and strategies. This chapter describes a protocol for the isolation and culture of primary mouse aortic ECs based on antibody-mediated EC selection.es_ES
dc.description.sponsorshipWork in V.A.’s laboratory is supported by grants from the Spanish Ministry for Economy and Competitiveness (MINECO) (SAF2013-46663-R), the Fondo Europeo de Desarrollo Regional (FEDER), the Instituto de Salud Carlos III (RD12/0042/0028), the Progeria Research Foundation (Innovator Award 2012, Established Investigator Award 2014), and the European Union (Liphos, Grant Agreement 317916). P.M-S. is supported by an FPU predoctoral fellowship from MINECO. The Centro Nacional de Investigaciones Cardiovasculares (CNIC) is supported by the MINECO and the Pro-CNIC Foundation.es_ES
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.relation.ispartofseriesMethods in Molelcular Biologyes_ES
dc.relation.isversionofPostprintes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectAntibody-mediated selectiones_ES
dc.subjectAtherosclerosises_ES
dc.subjectCulturees_ES
dc.subjectEndothelial cellses_ES
dc.subjectIsolationes_ES
dc.subjectMouse aortaes_ES
dc.subject.meshAnimals es_ES
dc.subject.meshAorta es_ES
dc.subject.meshAortic Diseases es_ES
dc.subject.meshAtherosclerosis es_ES
dc.subject.meshCells, Cultured es_ES
dc.subject.meshEndothelial Cells es_ES
dc.subject.meshImmunomagnetic Separation es_ES
dc.subject.meshMice es_ES
dc.subject.meshPrimary Cell Culture es_ES
dc.titleIsolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodieses_ES
dc.typeCapítulo de libroes_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID26445784
dc.format.volume1339
dc.format.page117
dc.identifier.doi10.1007/978-1-4939-2929-0_7es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderEuropean Regional Development Fund (ERDF/FEDER)es_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.contributor.funderProgeria Research Foundationes_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderFundación ProCNICes_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1940-6029
dc.relation.ispartofseriesnumber1339es_ES
dc.relation.publisherversionhttps://doi.org/10.1007/978-1-4939-2929-0_7es_ES
dc.identifier.journalMethods in molecular biology (Clifton, N.J.)
dc.repisalud.orgCNICCNIC::Grupos de investigación::Fisiopatología Cardiovascular Molecular y Genéticaes_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2013-46663-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD12/0042/0028es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/317916/EUes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución-NoComercial-CompartirIgual 4.0 Internacional
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