Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/7771
Título
RNA-based tools for nuclear reprogramming and lineage-conversion: towards clinical applications
Autor(es)
Bernal, Juan Antonio CNIC
Fecha de publicación
2013-12
Cita
J Cardiovasc Transl Res. 2013; 6(6):956-68
Idioma
Inglés
Tipo de documento
journal article
Resumen
The therapeutic potential of induced pluripotent stem cells (iPSCs) is well established. Safety concerns remain, however, and these have driven considerable efforts aimed at avoiding host genome alteration during the reprogramming process. At present, the tools used to generate human iPSCs include (1) DNA-based integrative and non-integrative methods and (2) DNA-free reprogramming technologies, including RNA-based approaches. Because of their combined efficiency and safety characteristics, RNA-based methods have emerged as the most promising tool for future iPSC-based regenerative medicine applications. Here, I will discuss novel recent advances in reprogramming technology, especially those utilizing the Sendai virus (SeV) and synthetic modified mRNA. In the future, these technologies may find utility in iPSC reprogramming for cellular lineage-conversion, and its subsequent use in cell-based therapies.
MESH
Animals | Cell Differentiation | Cell Lineage | Cellular Reprogramming | Epigenesis, Genetic | Gene Expression Regulation, Developmental | Genetic Vectors | Humans | Induced Pluripotent Stem Cells | MicroRNAs | RNA | RNA, Messenger | Sendai virus | Transcription Factors | Gene Transfer Techniques
Versión en línea
DOI
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