Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/7719
Título
iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications
Autor(es)
Fernandez-Chacon, Macarena CNIC | Casquero-Garcia, Veronica CNIC | Luo, Wen CNIC | Lunella, Federica Francesca CNIC | Rocha, Susana CNIC | Del Olmo-Cabrera, Sergio CNIC | Benedito, Rui CNIC
Fecha de publicación
2019
Cita
Nat Commun. 2019; 10(1):2262
Idioma
Inglés
Tipo de documento
journal article
Resumen
Most biomedical research aimed at understanding gene function uses the Cre-Lox system, which consists of the Cre recombinase-dependent deletion of genes containing LoxP sites. This system enables conditional genetic modifications because the expression and activity of the recombinase Cre/CreERT2 can be regulated in space by tissue-specific promoters and in time by the ligand tamoxifen. Since the precise Cre-Lox recombination event is invisible, methods were developed to report Cre activity and are widely used. However, numerous studies have shown that expression of a given Cre activity reporter cannot be assumed to indicate deletion of other LoxP-flanked genes of interest. Here, we report the generation of an inducible dual reporter-Cre mouse allele, iSuRe-Cre. By significantly increasing Cre activity in reporter-expressing cells, iSuRe-Cre provides certainty that these cells have completely recombined floxed alleles. This genetic tool increases the ease, efficiency, and reliability of conditional mutagenesis and gene function analysis.
Versión en línea
DOI
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- Nombre:
- iSuRe-CreIsAGeneticTool_2019_E ...
- Tamaño:
- 9.342Mb
- Formato:
- Microsoft Excel 2007