Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/7719
iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications
Fernandez-Chacon, Macarena CNIC | Casquero-Garcia, Veronica CNIC | Luo, Wen CNIC | Lunella, Federica Francesca CNIC | Rocha, Susana CNIC | Del Olmo-Cabrera, Sergio CNIC | Benedito, Rui CNIC
Nat Commun. 2019; 10(1):2262
Most biomedical research aimed at understanding gene function uses the Cre-Lox system, which consists of the Cre recombinase-dependent deletion of genes containing LoxP sites. This system enables conditional genetic modifications because the expression and activity of the recombinase Cre/CreERT2 can be regulated in space by tissue-specific promoters and in time by the ligand tamoxifen. Since the precise Cre-Lox recombination event is invisible, methods were developed to report Cre activity and are widely used. However, numerous studies have shown that expression of a given Cre activity reporter cannot be assumed to indicate deletion of other LoxP-flanked genes of interest. Here, we report the generation of an inducible dual reporter-Cre mouse allele, iSuRe-Cre. By significantly increasing Cre activity in reporter-expressing cells, iSuRe-Cre provides certainty that these cells have completely recombined floxed alleles. This genetic tool increases the ease, efficiency, and reliability of conditional mutagenesis and gene function analysis.