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dc.contributor.authorMartinez-Pinna, Roxana
dc.contributor.authorLopez, Juan Antonio 
dc.contributor.authorRamos-Mozo, Priscila
dc.contributor.authorBlanco-Colio, Luis M
dc.contributor.authorCamafeita, Emilio 
dc.contributor.authorCalvo, Enrique 
dc.contributor.authorMeilhac, Olivier
dc.contributor.authorMichel, Jean Baptiste
dc.contributor.authorEgido, Jesús
dc.contributor.authorMartin-Ventura, José Luis
dc.date.accessioned2019-05-23T13:49:11Z
dc.date.available2019-05-23T13:49:11Z
dc.date.issued2013
dc.identifier.citationMethods Mol Biol. 2013; 1000:91-101es_ES
dc.identifier.issn1064-3745es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7671
dc.description.abstractIn the search for novel biomarkers, noncandidate-based proteomic strategies open up new opportunities to gain a deeper insight into disease processes regarding their molecular mechanisms, the risk factors involved, and the monitoring of disease progression. To carry out these complex analyses, the combined use of gel electrophoresis with mass spectrometry (MS) represents a powerful choice. In addition, the introduction of protein dye labeling has notably improved the reliability of differential expression studies by increasing the statistical significance of the protein candidates. Here, we describe a strategy where different layers (luminal/abluminal) from the intraluminal thrombus (ILT) of human abdominal aortic aneurysm (AAA) patients were incubated in protein-free medium. Then, the levels of the proteins released were compared by two-dimensional differential in-gel electrophoresis (2D-DIGE) and the proteins of interest identified by MS. We consider that the use of tissue-conditioned media could offer a substantial advantage in the analytical study of biological fluids, as they provide a source of proteins to be released to the bloodstream, which could serve as potential circulating biomarkers.es_ES
dc.description.sponsorshipThis chapter has been supported by the EC, FAD project (FP-7, HEALTH F2-2008-200647), the Spanish MICIN (SAF2010/21852), Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Redes RECAVA (RD06/0014/0035), EUS2008-03565, and Fundacion Pro CNIC.es_ES
dc.language.isoenges_ES
dc.relation.isversionofPostprintes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.meshAortic Aneurysm, Abdominal es_ES
dc.subject.meshBiomarkers es_ES
dc.subject.meshData Mining es_ES
dc.subject.meshDatabases, Proteines_ES
dc.subject.meshElectrophoresis, Gel, Two-Dimensional es_ES
dc.subject.meshHumans es_ES
dc.subject.meshProteolysis es_ES
dc.subject.meshProteomics es_ES
dc.subject.meshSilver Staining es_ES
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationes_ES
dc.subject.meshThrombosis es_ES
dc.subject.meshTrypsin es_ES
dc.subject.meshCulture Media, Conditioned es_ES
dc.titleIdentification of novel biomarkers of abdominal aortic aneurysms by 2D-DIGE and MALDI-MS from AAA-thrombus-conditioned mediaes_ES
dc.typeArtículoes_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID23585087es_ES
dc.format.volume1000es_ES
dc.format.page91-101es_ES
dc.identifier.doi10.1007/978-1-62703-405-0_8es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderMinisterio de Sanidad y Consumo (España)es_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderFundación ProCNICes_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1940-6029es_ES
dc.relation.publisherversionhttps://doi.org/10.1007/978-1-62703-405-0_8es_ES
dc.identifier.journalMethods in molecular biology (Clifton, N.J.)es_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Proteómica / Metabolómicaes_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/200647/EUes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2010/21852es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD06/0014/0035es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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