dc.contributor.author | Martinez-Pinna, Roxana | |
dc.contributor.author | Lopez, Juan Antonio | |
dc.contributor.author | Ramos-Mozo, Priscila | |
dc.contributor.author | Blanco-Colio, Luis M | |
dc.contributor.author | Camafeita, Emilio | |
dc.contributor.author | Calvo, Enrique | |
dc.contributor.author | Meilhac, Olivier | |
dc.contributor.author | Michel, Jean Baptiste | |
dc.contributor.author | Egido, Jesús | |
dc.contributor.author | Martin-Ventura, José Luis | |
dc.date.accessioned | 2019-05-23T13:49:11Z | |
dc.date.available | 2019-05-23T13:49:11Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Methods Mol Biol. 2013; 1000:91-101 | es_ES |
dc.identifier.issn | 1064-3745 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/7671 | |
dc.description.abstract | In the search for novel biomarkers, noncandidate-based proteomic strategies open up new opportunities to gain a deeper insight into disease processes regarding their molecular mechanisms, the risk factors involved, and the monitoring of disease progression. To carry out these complex analyses, the combined use of gel electrophoresis with mass spectrometry (MS) represents a powerful choice. In addition, the introduction of protein dye labeling has notably improved the reliability of differential expression studies by increasing the statistical significance of the protein candidates. Here, we describe a strategy where different layers (luminal/abluminal) from the intraluminal thrombus (ILT) of human abdominal aortic aneurysm (AAA) patients were incubated in protein-free medium. Then, the levels of the proteins released were compared by two-dimensional differential in-gel electrophoresis (2D-DIGE) and the proteins of interest identified by MS. We consider that the use of tissue-conditioned media could offer a substantial advantage in the analytical study of biological fluids, as they provide a source of proteins to be released to the bloodstream, which could serve as potential circulating biomarkers. | es_ES |
dc.description.sponsorship | This chapter has been supported by the EC, FAD project (FP-7, HEALTH F2-2008-200647), the Spanish MICIN (SAF2010/21852), Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Redes RECAVA (RD06/0014/0035), EUS2008-03565, and Fundacion Pro CNIC. | es_ES |
dc.language.iso | eng | es_ES |
dc.type.hasVersion | AM | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject.mesh | Aortic Aneurysm, Abdominal | es_ES |
dc.subject.mesh | Biomarkers | es_ES |
dc.subject.mesh | Data Mining | es_ES |
dc.subject.mesh | Databases, Protein | es_ES |
dc.subject.mesh | Electrophoresis, Gel, Two-Dimensional | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Proteolysis | es_ES |
dc.subject.mesh | Proteomics | es_ES |
dc.subject.mesh | Silver Staining | es_ES |
dc.subject.mesh | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | es_ES |
dc.subject.mesh | Thrombosis | es_ES |
dc.subject.mesh | Trypsin | es_ES |
dc.subject.mesh | Culture Media, Conditioned | es_ES |
dc.title | Identification of novel biomarkers of abdominal aortic aneurysms by 2D-DIGE and MALDI-MS from AAA-thrombus-conditioned media | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.identifier.pubmedID | 23585087 | es_ES |
dc.format.volume | 1000 | es_ES |
dc.format.page | 91-101 | es_ES |
dc.identifier.doi | 10.1007/978-1-62703-405-0_8 | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | |
dc.contributor.funder | Ministerio de Sanidad y Consumo (España) | |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Unión Europea. Comisión Europea | |
dc.contributor.funder | Fundación ProCNIC | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1940-6029 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1007/978-1-62703-405-0_8 | es_ES |
dc.identifier.journal | Methods in molecular biology (Clifton, N.J.) | es_ES |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Proteómica / Metabolómica | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/200647/EU | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2010/21852 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RD06/0014/0035 | es_ES |
dc.rights.accessRights | open access | es_ES |