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dc.contributor.authorGarrido-Gomez, Tamara
dc.contributor.authorDominguez, Francisco 
dc.contributor.authorLopez, Juan Antonio 
dc.contributor.authorCamafeita, Emilio 
dc.contributor.authorQuiñonero, Alicia
dc.contributor.authorMartinez-Conejero, Jose Antonio
dc.contributor.authorPellicer, Antonio
dc.contributor.authorConesa, Ana
dc.contributor.authorSimón, Carlos
dc.date.accessioned2019-05-23T13:38:17Z
dc.date.available2019-05-23T13:38:17Z
dc.date.issued2011-03
dc.identifier.citationJ Clin Endocrinol Metab. 2011; 3(96):706-16es_ES
dc.identifier.issn0021-972Xes_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7670
dc.description.abstractCONTEXT: Decidualization of the human endometrium, which involves morphological and biochemical modifications of the endometrial stromal cells (ESCs), is a prerequisite for adequate trophoblast invasion and placenta formation. OBJECTIVE: This study aims to investigate the proteome and secretome of in vitro decidualized ESCs. These data were combined with published genomic information and integrated to model the human decidualization interactome. DESIGN: Prospective experimental case-control study. SETTING: A private research foundation. PATIENTS: Sixteen healthy volunteer ovum donors. INTERVENTION: Endometrial samples were obtained, and ESCs were isolated and decidualized in vitro. MAIN OUTCOME MEASURES: Two-dimensional difference in-gel electrophoresis, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry, Western blot, human protein cytokine array, ELISA, and bioinformatics analysis were performed. RESULTS: The proteomic analysis revealed 60 differentially expressed proteins (36 over- and 24 underexpressed) in decidualized versus control ESCs, including known decidualization markers (cathepsin B) and new biomarkers (transglutaminase 2, peroxiredoxin 4, and the ACTB protein). In the secretomic analysis, a total of 13 secreted proteins (11 up- and 2 down-regulated) were identified, including well-recognized markers (IGF binding protein-1 and prolactin) and novel ones (myeloid progenitor inhibitory factor-1 and platelet endothelial cell adhesion molecule-1). These proteome/secretome profiles have been integrated into a decidualization interactome model. CONCLUSIONS: Proteomic and secretomic have been used as hypothesis-free approaches together with complex bioinformatics to model the human decidual interactome for the first time. We confirm previous knowledge, describe new molecules, and we have built up a model for human in vitro decidualization as invaluable tool for the diagnosis, therapy, and interpretation of biological phenomena.es_ES
dc.description.sponsorshipThe participation of T.G.-G. was supported by a grant of the Ministry of Education and Science AP2007-04066 (FPU). CNIC was supported by the Spanish Ministry of Science and Innovation and the Pro-CNIC Foundation.es_ES
dc.language.isoenges_ES
dc.publisherOxford University Press es_ES
dc.type.hasVersionAMes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.meshAdolescent es_ES
dc.subject.meshAdult es_ES
dc.subject.meshBiomarkers es_ES
dc.subject.meshBlotting, Westernes_ES
dc.subject.meshCase-Control Studies es_ES
dc.subject.meshCell Shape es_ES
dc.subject.meshCells, Cultured es_ES
dc.subject.meshComputational Biology es_ES
dc.subject.meshCulture Media, Conditioned es_ES
dc.subject.meshCytokines es_ES
dc.subject.meshDecidua es_ES
dc.subject.meshElectrophoresis, Polyacrylamide Gel es_ES
dc.subject.meshEndometrium es_ES
dc.subject.meshEnzyme-Linked Immunosorbent Assay es_ES
dc.subject.meshFemale es_ES
dc.subject.meshHumans es_ES
dc.subject.meshModels, Biologicales_ES
dc.subject.meshProtein Processing, Post-Translationales_ES
dc.subject.meshProteome es_ES
dc.subject.meshProteomics es_ES
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationes_ES
dc.subject.meshStromal Cells es_ES
dc.subject.meshYoung Adult es_ES
dc.titleModeling human endometrial decidualization from the interaction between proteome and secretomees_ES
dc.typejournal articlees_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID21190976es_ES
dc.format.volume96es_ES
dc.format.number3es_ES
dc.format.page706-16es_ES
dc.identifier.doi10.1210/jc.2010-1825es_ES
dc.contributor.funderMinisterio de Educación y Ciencia (España) 
dc.contributor.funderMinisterio de Ciencia e Innovación (España) 
dc.contributor.funderFundación ProCNIC 
dc.description.peerreviewedes_ES
dc.identifier.e-issn1945-7197es_ES
dc.relation.publisherversionhttps://doi.org/10.1210/jc.2010-1825es_ES
dc.identifier.journalThe Journal of clinical endocrinology and metabolismes_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Proteómica / Metabolómicaes_ES
dc.repisalud.institucionCNICes_ES
dc.rights.accessRightsopen accesses_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Attribution-NonCommercial-NoDerivatives 4.0 Internacional