Show simple item record

dc.contributor.authorRamos-Mozo, Priscila
dc.contributor.authorMadrigal-Matute, Julio
dc.contributor.authorMartinez-Pinna, Roxana
dc.contributor.authorBlanco-Colio, Luis Miguel
dc.contributor.authorLopez, Juan Antonio 
dc.contributor.authorCamafeita, Emilio 
dc.contributor.authorMeilhac, Olivier
dc.contributor.authorMichel, Jean-Baptiste
dc.contributor.authorAparicio, Cesar
dc.contributor.authorVega de Ceniga, Melina
dc.contributor.authorEgido, Jesus
dc.contributor.authorMartín-Ventura, José Luis
dc.identifier.citationArterioscler Thromb Vasc Biol. 2011; 31(12):3011-9es_ES
dc.description.abstractOBJECTIVE: Polymorphonuclear neutrophils (PMNs) play a main role in abdominal aortic aneurysm (AAA) progression. We have analyzed circulating PMNs isolated from AAA patients and controls by a proteomic approach to identify proteins potentially involved in AAA pathogenesis. METHODS AND RESULTS: PMNs from 8 AAA patients (4 large AAA >5 cm and 4 small AAA 3-5 cm) and 4 controls were analyzed by 2D differential in-gel electrophoresis. Among differentially expressed spots, several proteins involved in redox balance were identified by mass spectrometry (eg, cyclophilin, thioredoxin reductase, catalase). Diminished catalase expression and activity were observed in PMNs from AAA patients compared with controls. In contrast, PMNs from AAA patients displayed higher H(2)O(2) and myeloperoxidase levels than PMNs from controls. Moreover, a significant decrease in catalase mRNA levels was observed in PMNs after phorbol 12-myristate 13-acetate incubation. Catalase plasma levels were also decreased in large (n=47) and small (n=56) AAA patients compared with controls (n=34). We observed catalase expression in AAA thrombus and thrombus-conditioned medium, associated with PMN infiltration. Furthermore, increased H(2)O(2) levels were observed in AAA thrombus-conditioned medium compared with the media layer. CONCLUSIONS: Diminished catalase levels in circulating PMNs and plasma are observed in AAA patients, supporting an important role of oxidative stress in AAA evolution.es_ES
dc.description.sponsorshipThis work was supported by the European Commission, Fighting Aneurysmal Disease project (FP-7, HEALTH F2-2008-200647), the Spanish Ministerio de Ciencia e Innovacion (SAF2010/21852), Fundacion Ramon Areces, Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Redes RECAVA (RD06/0014/0035), EUS2008-03565, and Fundacion Pro CNIC.es_ES
dc.subject.meshAged es_ES
dc.subject.meshAged, 80 and over es_ES
dc.subject.meshAortic Aneurysm, Abdominal es_ES
dc.subject.meshBiomarkers es_ES
dc.subject.meshCase-Control Studies es_ES
dc.subject.meshCatalase es_ES
dc.subject.meshElectrophoresis, Gel, Two-Dimensional es_ES
dc.subject.meshFemale es_ES
dc.subject.meshHumans es_ES
dc.subject.meshHydrogen Peroxide es_ES
dc.subject.meshMale es_ES
dc.subject.meshMiddle Aged es_ES
dc.subject.meshNeutrophils es_ES
dc.subject.meshOxidation-Reduction es_ES
dc.subject.meshOxidative Stress es_ES
dc.subject.meshPeroxidase es_ES
dc.subject.meshDisease Progression es_ES
dc.subject.meshProteomics es_ES
dc.titleProteomic analysis of polymorphonuclear neutrophils identifies catalase as a novel biomarker of abdominal aortic aneurysm: potential implication of oxidative stress in abdominal aortic aneurysm progressiones_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderUnión Europea. Comisión Europea 
dc.contributor.funderMinisterio de Ciencia e Innovación (España) 
dc.contributor.funderFundación Ramón Areces 
dc.contributor.funderMinisterio de Sanidad y Consumo (España) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderFundación ProCNIC 
dc.identifier.journalArteriosclerosis, thrombosis, and vascular biologyes_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Proteómica / Metabolómicaes_ES
dc.rights.accessRightsopen accesses_ES

Files in this item

Acceso Abierto

This item appears in the following Collection(s)

Show simple item record

Atribución-NoComercial-CompartirIgual 4.0 Internacional
This item is licensed under a: Atribución-NoComercial-CompartirIgual 4.0 Internacional