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dc.contributor.author | Rodriguez-Mahillo, Ana Isabel | |
dc.contributor.author | Gonzalez-Muñoz, Miguel | |
dc.contributor.author | Vega, José María | |
dc.contributor.author | Lopez, Juan Antonio | |
dc.contributor.author | Yart, Annie | |
dc.contributor.author | Kerdelhué, Carole | |
dc.contributor.author | Camafeita, Emilio | |
dc.contributor.author | Garcia Ortiz, José Carlos | |
dc.contributor.author | Vogel, Heiko | |
dc.contributor.author | Petrucco Toffolo, Edoardo | |
dc.contributor.author | Zovi, Daniel | |
dc.contributor.author | Battisti, Andrea | |
dc.contributor.author | Roques, Alain | |
dc.contributor.author | Moneo, Ignacio | |
dc.date.accessioned | 2019-05-23T12:32:56Z | |
dc.date.available | 2019-05-23T12:32:56Z | |
dc.date.issued | 2012-12 | |
dc.identifier.citation | Contact Dermatitis. 2012; 67(6):367-74 | es_ES |
dc.identifier.issn | 01051873 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/7667 | |
dc.description.abstract | BACKGROUND: Pine processionary larvae produce urticating hairs (setae) that serve for protection against predators. Setae induce cutaneous reactions in animals and humans. The presence of toxic or allergic mechanisms is a matter of debate. OBJECTIVES: To detect the presence of allergens in setae and to characterize them. MATERIALS AND METHODS: Setae extracts were characterized by gel staining and immunoblot, with sera from patients with immediate reactions and positive prick test reactions, as well as a rabbit antiserum raised against setae. Setae proteins were fractionated by high-performance liquid chromatography. The most relevant allergen was analysed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), and its sequence was deduced from an expressed sequence tag bank. Results. Setae contained at least seven different allergens. The most intense detection corresponded to a protein of MW ~ 14,000 that was similar to thaumetopoein, a previously described protein with mast cell-degranulating properties. MALDI-MS-based de novo sequencing provided a partial amino acid sequence different from that of the previously described allergen Tha p 1, and it was named Tha p 2. This allergen was detected in 61% of patients, and it is therefore a new major caterpillar allergen. CONCLUSIONS: Penetration of the setae from the pine processionary caterpillar delivers their allergenic content in addition to causing mechanical or toxic injury. | es_ES |
dc.description.sponsorship | This work was supported by URTICLIM, a French project funded by the ‘Agence Nationale de la Recherche’ (ANR 07BDIV 013). Ana I. Rodriguez-Mahillo is a beneficiary of contract CA07/00046 from the Instituto de Salud Carlos III, Ministerio de Ciencia e Innovacion, Spain. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.type.hasVersion | AM | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject.mesh | Allergens | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Antibodies, Antinuclear | es_ES |
dc.subject.mesh | Dermatitis, Allergic Contact | es_ES |
dc.subject.mesh | Electrophoresis, Polyacrylamide Gel | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Lepidoptera | es_ES |
dc.subject.mesh | Moths | es_ES |
dc.subject.mesh | Patch Tests | es_ES |
dc.subject.mesh | Sensitivity and Specificity | es_ES |
dc.subject.mesh | Spain | es_ES |
dc.title | Setae from the pine processionary moth (Thaumetopoea pityocampa) contain several relevant allergens | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.identifier.pubmedID | 22708991 | es_ES |
dc.format.volume | 67 | es_ES |
dc.format.number | 6 | es_ES |
dc.format.page | 367-74 | es_ES |
dc.identifier.doi | 10.1111/j.1600-0536.2012.02107.x | es_ES |
dc.contributor.funder | Agence Nationale de la Recherche (Francia) | |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1600-0536 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1111/j.1600-0536.2012.02107.x | es_ES |
dc.identifier.journal | Contact dermatitis | es_ES |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Proteómica / Metabolómica | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/CA07/00046 | es_ES |
dc.rights.accessRights | open access | es_ES |