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dc.contributor.author | Zamai, Moreno | |
dc.contributor.author | Trullo, Antonio | |
dc.contributor.author | Giordano, Marco | |
dc.contributor.author | Corti, Valeria | |
dc.contributor.author | Arza, Elvira | |
dc.contributor.author | Francavilla, Chiara | |
dc.contributor.author | Cavallaro, Ugo | |
dc.contributor.author | Caiolfa, Valeria R | |
dc.date.accessioned | 2019-05-08T05:55:04Z | |
dc.date.available | 2019-05-08T05:55:04Z | |
dc.date.issued | 2019-01-03 | |
dc.identifier.citation | J Cell Sci. 2019; 132(1):jcs220624 | es_ES |
dc.identifier.issn | 0021-9533 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/7551 | |
dc.description.abstract | Both fibroblast growth factor-2 (FGF2) and neural cell adhesion molecule (NCAM) trigger FGF receptor 1 (FGFR1) signaling; however, they induce remarkably distinct receptor trafficking and cellular responses. The molecular basis of such a dichotomy and the role of distinct types of ligand-receptor interaction remain elusive. Number of molecules and brightness (N&B) analysis revealed that FGF2 and NCAM promote different FGFR1 assembly and dynamics at the plasma membrane. NCAM stimulation elicits long-lasting cycles of short-lived FGFR1 monomers and multimers, a behavior that might reflect a rapid FGFR1 internalization and recycling. FGF2, instead, induces stable dimerization at the dose that stimulates cell proliferation. Reducing the occupancy of FGFR1 in response to low FGF2 doses causes a switch towards cyclically exposed and unstable receptor dimers, consistently with previously reported biphasic response to FGF2 and with the divergent signaling elicited by different ligand concentrations. Similar instability was observed upon altering the endocytic pathway. Thus, FGF2 and NCAM induce differential FGFR1 clustering at the cell surface, which might account for the distinct intracellular fate of the receptor and, hence, for the different signaling cascades and cellular responses. | es_ES |
dc.description.sponsorship | U.C. and V.R.C. acknowledge the support of Fondazione CARIPLO, Milano (Grant 2375 2009-2012). U.C. also acknowledges the support from the Associazione Italiana Ricerca sul Cancro, the Association for International Cancer Research (now known as Worldwide Cancer Research), and the Italian Ministry of Health. A. T. acknowledges the 'Fondazione Banca del Monte di Lombardia' for partly supporting his work with the PV Fellowship 'Progetto Professionalita Ivano Becchi' (20112012). M.G. was supported by a fellowship from Fondazione IEO-CCM. The CNIC is supported by the Ministry of Ciencia, Innovaciòn y Universidades and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV-2015-0505). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | The Company of Biologists | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | FGF | es_ES |
dc.subject | FGFR1 | es_ES |
dc.subject | FGFR1 clustering | es_ES |
dc.subject | FGFR1 signaling | es_ES |
dc.subject | N&B analysis | es_ES |
dc.subject | NCAM | es_ES |
dc.subject | Receptor clustering | es_ES |
dc.title | Number and brightness analysis reveals that NCAM and FGF2 elicit different assembly and dynamics of FGFR1 in live cells | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.identifier.pubmedID | 30478195 | es_ES |
dc.format.volume | 132 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | jcs220624 | es_ES |
dc.identifier.doi | 10.1242/jcs.220624 | es_ES |
dc.contributor.funder | Fondazione Cariplo | |
dc.contributor.funder | Italian Association for Cancer Research | |
dc.contributor.funder | Worldwide Cancer Research | |
dc.contributor.funder | Fondazione Banca del Monte di Lombardia | |
dc.contributor.funder | Fondazione IEO-CCM | |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | |
dc.contributor.funder | Fundación ProCNIC | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1477-9137 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1242/jcs.220624 | es_ES |
dc.identifier.journal | Journal of cell science | es_ES |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Microscopía | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SEV-2015-0505 | es_ES |
dc.rights.accessRights | open access | es_ES |