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dc.contributor.authorMessina, Giovanni
dc.contributor.authorAtterrato, Maria Teresa
dc.contributor.authorProzzillo, Yuri
dc.contributor.authorPiacentini, Lucia
dc.contributor.authorLosada, Ana 
dc.contributor.authorDimitri, Patrizio
dc.date.accessioned2019-03-07T11:48:14Z
dc.date.available2019-03-07T11:48:14Z
dc.date.issued2017-04-03
dc.identifier.citationSci Rep. 2017;7:45022.es_ES
dc.identifier.issn2045-2322es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7299
dc.description.abstractThe human Cranio Facial Development Protein 1 (Cfdp1) gene maps to chromosome 16q22.2-q22.3 and encodes the CFDP1 protein, which belongs to the evolutionarily conserved Bucentaur (BCNT) family. Craniofacial malformations are developmental disorders of particular biomedical and clinical interest, because they represent the main cause of infant mortality and disability in humans, thus it is important to understand the cellular functions and mechanism of action of the CFDP1 protein. We have carried out a multi-disciplinary study, combining cell biology, reverse genetics and biochemistry, to provide the first in vivo characterization of CFDP1 protein functions in human cells. We show that CFDP1 binds to chromatin and interacts with subunits of the SRCAP chromatin remodeling complex. An RNAi-mediated depletion of CFDP1 in HeLa cells affects chromosome organization, SMC2 condensin recruitment and cell cycle progression. Our findings provide new insight into the chromatin functions and mechanisms of the CFDP1 protein and contribute to our understanding of the link between epigenetic regulation and the onset of human complex developmental disorders.es_ES
dc.description.sponsorshipWe are grateful to Tatsuya Hirano for gift of ISWI and H3p antibodies and to Ana Cuadrado for gift of anti-p18Hamlet antibody, pCS2-5xMyc-Arp6, pcDNA3-HA-H2A.Z and pcDNA3-HA-SRCAP plasmids.es_ES
dc.language.isoenges_ES
dc.publisherNature Publishing Group es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAdenosine Triphosphatases es_ES
dc.subject.meshCell Line es_ES
dc.subject.meshChromatin es_ES
dc.subject.meshChromatin Assembly and Disassembly es_ES
dc.subject.meshChromosomes es_ES
dc.subject.meshDNA-Binding Proteins es_ES
dc.subject.meshGene Expression es_ES
dc.subject.meshHeLa Cells es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMitosis es_ES
dc.subject.meshMultiprotein Complexes es_ES
dc.subject.meshPhosphoproteins es_ES
dc.subject.meshProtein Binding es_ES
dc.subject.meshProtein Interaction Domains and Motifs es_ES
dc.subject.meshProtein Transport es_ES
dc.titleThe human Cranio Facial Development Protein 1 (Cfdp1) gene encodes a protein required for the maintenance of higher-order chromatin organizationes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID28367969es_ES
dc.format.volume7es_ES
dc.format.number1es_ES
dc.format.page45022es_ES
dc.identifier.doi10.1038/srep45022es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn2045-2322es_ES
dc.relation.publisherversionhttps://doi.org/ 10.1038/srep45022.es_ES
dc.identifier.journalScientific reportses_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Dinámica Cromosómicaes_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
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