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dc.contributor.authorRey, María-Dolores
dc.contributor.authorCastillejo, María Ángeles
dc.contributor.authorSánchez-Lucas, Rosa
dc.contributor.authorGuerrero-Sanchez, Victor M
dc.contributor.authorLópez-Hidalgo, Cristina
dc.contributor.authorRomero-Rodríguez, Cristina
dc.contributor.authorValero-Galván, José
dc.contributor.authorSghaier-Hammami, Besma
dc.contributor.authorSimova-Stoilova, Lyudmila
dc.contributor.authorEchevarría-Zomeño, Sira
dc.contributor.authorJorge, Inmaculada 
dc.contributor.authorGómez-Gálvez, Isabel
dc.contributor.authorPapa, María Eugenia
dc.contributor.authorCarvalho, Kamilla
dc.contributor.authorRodríguez de Francisco, Luis E
dc.contributor.authorMaldonado-Alconada, Ana María
dc.contributor.authorValledor, Luis
dc.contributor.authorJorrín-Novo, Jesús V
dc.identifier.citationInt J Mol Sci. 2019; 20(3):692es_ES
dc.description.abstractProteomics has had a big impact on plant biology, considered as a valuable tool for several forest species, such as Quercus, Pines, Poplars, and Eucalyptus. This review assesses the potential and limitations of the proteomics approaches and is focused on Quercus ilex as a model species and other forest tree species. Proteomics has been used with Q. ilex since 2003 with the main aim of examining natural variability, developmental processes, and responses to biotic and abiotic stresses as in other species of the genus Quercus or Pinus. As with the progress in techniques in proteomics in other plant species, the research in Q. ilex moved from 2-DE based strategy to the latest gel-free shotgun workflows. Experimental design, protein extraction, mass spectrometric analysis, confidence levels of qualitative and quantitative proteomics data, and their interpretation are a true challenge with relation to forest tree species due to their extreme orphan and recalcitrant (non-orthodox) nature. Implementing a systems biology approach, it is time to validate proteomics data using complementary techniques and integrate it with the -omics and classical approaches. The full potential of the protein field in plant research is quite far from being entirely exploited. However, despite the methodological limitations present in proteomics, there is no doubt that this discipline has contributed to deeper knowledge of plant biology and, currently, is increasingly employed for translational purposes.es_ES
dc.description.sponsorshipThis research was funded by the Ministerio de Economía y Competitividad -Programa Estatal de I+D+i Orientada a los Retos de la Sociedad (AGL2009-12243-C02-02; BIO2015-64737-R).es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subject2-DE proteomicses_ES
dc.subjectQuercus ilexes_ES
dc.subjectholm oakes_ES
dc.subjectnon-orthodox seedes_ES
dc.subjectpopulation variabilityes_ES
dc.subjectshotgun proteomicses_ES
dc.subjectstresses responseses_ES
dc.titleProteomics, Holm Oak (Quercus ilex L.) and Other Recalcitrant and Orphan Forest Tree Species: How do They See Each Other?es_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.identifier.journalInternational journal of molecular scienceses_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovasculares_ES

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