Show simple item record

dc.contributor.authorMarión, Rosa M
dc.contributor.authorLopez de Silanes, Isabel 
dc.contributor.authorMosteiro, Lluc
dc.contributor.authorGamache, Benjamin
dc.contributor.authorAbad, María
dc.contributor.authorGuerra Carmen, Carmen 
dc.contributor.authorMegias Vazquez, Diego 
dc.contributor.authorSerrano Marugan, Manuel 
dc.contributor.authorBlasco , MA 
dc.date.accessioned2019-02-26T12:07:39Z
dc.date.available2019-02-26T12:07:39Z
dc.date.issued2017-02-14
dc.identifier.citationStem Cell Reports. 2017;8(2):460-475.es_ES
dc.identifier.issn22136711es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7237
dc.description.abstractReprogramming of differentiated cells into induced pluripotent stem cells has been recently achieved in vivo in mice. Telomeres are essential for chromosomal stability and determine organismal life span as well as cancer growth. Here, we study whether tissue dedifferentiation induced by in vivo reprogramming involves changes at telomeres. We find telomerase-dependent telomere elongation in the reprogrammed areas. Notably, we found highly upregulated expression of the TRF1 telomere protein in the reprogrammed areas, which was independent of telomere length. Moreover, TRF1 inhibition reduced in vivo reprogramming efficiency. Importantly, we extend the finding of TRF1 upregulation to pathological tissue dedifferentiation associated with neoplasias, in particular during pancreatic acinar-to-ductal metaplasia, a process that involves transdifferentiation of adult acinar cells into ductal-like cells due to K-Ras oncogene expression. These findings place telomeres as important players in cellular plasticity both during in vivo reprogramming and in pathological conditions associated with increased plasticity, such as cancer.es_ES
dc.description.sponsorshipWe are indebted to R. Serrano for expert mouse colony manage- ment and the Comparative Pathology Unit at CNIO for technical assistance. We are grateful to Dr. Ana Losada for providing us with SA1 antibody. We thank Ana Carolina Moises da Silva for her assistance in setting-up RNA-FISH experiments. Work in the laboratory of M.A.B. is funded by the Spanish Ministry of Economy and Competiveness (PLAN RETOS SAF2013-45111-R) and by the Fundacion Botın.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectADMes_ES
dc.subjectTRF1es_ES
dc.subjectcanceres_ES
dc.subjectcellular plasticityes_ES
dc.subjectin vivo reprogramminges_ES
dc.subjectregenerationes_ES
dc.subjectstem cellses_ES
dc.subjecttelomereses_ES
dc.subjecttransdifferentiationes_ES
dc.subjecttumorigenesises_ES
dc.subject.meshAnimals es_ES
dc.subject.meshCell Cycle Proteins es_ES
dc.subject.meshCell Dedifferentiation es_ES
dc.subject.meshCell Transformation, Neoplastices_ES
dc.subject.meshCellular Reprogramming es_ES
dc.subject.meshChromatin Assembly and Disassembly es_ES
dc.subject.meshChromosomal Proteins, Non-Histonees_ES
dc.subject.meshGene Expression Regulation es_ES
dc.subject.meshHeterochromatin es_ES
dc.subject.meshMice es_ES
dc.subject.meshMice, Transgenic es_ES
dc.subject.meshProtein Subunits es_ES
dc.subject.meshStem Cells es_ES
dc.subject.meshTelomerase es_ES
dc.subject.meshTelomere es_ES
dc.subject.meshTelomere Homeostasis es_ES
dc.subject.meshTelomeric Repeat Binding Protein 1 es_ES
dc.titleCommon Telomere Changes during In Vivo Reprogramming and Early Stages of Tumorigenesises_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID28162998es_ES
dc.format.volume8es_ES
dc.format.number2es_ES
dc.format.page460-475es_ES
dc.identifier.doi10.1016/j.stemcr.2017.01.001es_ES
dc.contributor.funderFundación Marcelino Botínes_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn2213-6711es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.stemcr.2017.01.001.es_ES
dc.identifier.journalStem cell reportses_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Telómeros y Telomerasaes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2013-45111-Res_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


Files in this item

Acceso Abierto
Thumbnail
Acceso Abierto
Thumbnail
Acceso Abierto
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Atribución 4.0 Internacional
This item is licensed under a: Atribución 4.0 Internacional