Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/7129
Título
A predictive model of asymmetric morphogenesis from 3D reconstructions of mouse heart looping dynamics
Autor(es)
Fecha de publicación
2017
Cita
Elife. 2017; 6:e28951
Idioma
Inglés
Tipo de documento
journal article
Resumen
How left-right patterning drives asymmetric morphogenesis is unclear. Here, we have quantified shape changes during mouse heart looping, from 3D reconstructions by HREM. In combination with cell labelling and computer simulations, we propose a novel model of heart looping. Buckling, when the cardiac tube grows between fixed poles, is modulated by the progressive breakdown of the dorsal mesocardium. We have identified sequential left-right asymmetries at the poles, which bias the buckling in opposite directions, thus leading to a helical shape. Our predictive model is useful to explore the parameter space generating shape variations. The role of the dorsal mesocardium was validated in Shh-/- mutants, which recapitulate heart shape changes expected from a persistent dorsal mesocardium. Our computer and quantitative tools provide novel insight into the mechanism of heart looping and the contribution of different factors, beyond the simple description of looping direction. This is relevant to congenital heart defects.
Palabras clave
3D shape | Computer modelling | Developmental biology | Heart morphogenesis | Left-right patterning | Mouse | Stem cells
MESH
Animals | Computer Simulation | Heart | Imaging, Three-Dimensional | Mice | Microscopy | Morphogenesis
Versión en línea
DOI
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