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dc.contributor.authorGarcia, Marcial
dc.contributor.authorAngeles Navarrete-Munoz, Maria
dc.contributor.authorLigos, Jose M. 
dc.contributor.authorCabello, Alfonso
dc.contributor.authorRestrepo, Clara
dc.contributor.authorLopez-Bernaldo, Juan Carlos
dc.contributor.authorde la Hera, Francisco Javier
dc.contributor.authorBarros, Carlos
dc.contributor.authorMontoya, Maria 
dc.contributor.authorFernandez-Guerrero, Manuel
dc.contributor.authorEstrada, Vicente
dc.contributor.authorGorgolas, Miguel
dc.contributor.authorBenito, José Miguel
dc.contributor.authorRallon, Norma
dc.date.accessioned2018-11-22T08:10:49Z
dc.date.available2018-11-22T08:10:49Z
dc.date.issued2018
dc.identifierISI:000447707900056
dc.identifier.citationSci Rep. 2018; 8(1):15541
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6662
dc.description.abstractA recent study has pointed out to CD32a as a potential biomarker of HIV-persistent CD4 cells. We have characterized the level and phenotype of CD32+ cells contained in different subsets of CD4 T-cells and its potential correlation with level of total HIV-DNA in thirty HIV patients (10 typical progressors naive for cART, 10 cART-suppressed patients, and 10 elite controllers). Total HIV-DNA was quantified in different subsets of CD4 T-cells: Trm and pTfh cells. Level and immunephenotype of CD32+ cells were analyzed in these same subsets by flow cytometry. CD32 expression in Trm and pTfh subsets was similar in the different groups, and there was no significant correlation between the level of total HIV-DNA and the level of CD32 expression in these subsets. However, total HIV-DNA level was correlated with expression of CD127 (rho = -0.46, p = 0.043) and of CCR6 (rho = -0.418, p = 0.027) on CD32+ cells. Our results do not support CD32 as a biomarker of total HIV-DNA content. However, analyzing the expression of certain markers by CD32+ cells could improve the utility of this marker in the clinical setting, prompting the necessity of further studies to both validate our results and to explore the potential utility of certain markers expressed by CD32+ cells.
dc.description.sponsorshipWe would like to thank all patients and healthy donors who participated in the study. This study has been funded by projects CP14/00198, PI16/01769, and RD16/0025/0013 integrated in the State Plan for Scientific and Technical Research and Innovation and co-funded by ISCIII-Sub-Directorate General for Research Assessment and Promotion and European Regional Development Fund (ERDF). N Rallon is a Miguel Servet investigator from the Spanish Carlos III Institute of Health (ISCIII), grant CP14/00198, Madrid, Spain. Maria Angeles Navarrete-Munoz was funded by RD16/0025/0013 and the Intramural Research Scholarship from IIS-FJD. Clara Restrepo was funded by project RD16/0025/0013. M Garcia is a predoctoral student co-funded by CP14/00198 project and the Intramural Research Scholarship from IIS-FJD.
dc.language.isoeng
dc.publisherNature Publishing Group 
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectACTIVE ANTIRETROVIRAL THERAPY
dc.subjectHUMAN T-CELLS
dc.subjectLATENT RESERVOIR
dc.subjectINFECTED INDIVIDUALS
dc.subjectTH17 CELLS
dc.subjectPERSISTENCE
dc.subjectRECEPTORS
dc.subjectBLOOD
dc.subjectSUBPOPULATIONS
dc.subjectLYMPHOCYTES
dc.titleCD32 Expression is not Associated to HIV-DNA content in CD4 cell subsets of individuals with Different Levels of HIV Control
dc.typejournal article
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID30341387
dc.format.volume8
dc.identifier.doi10.1038/s41598-018-33749-5
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderInstituto de Investigación Sanitaria de la Fundación Jiménez Díaz 
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) 
dc.description.peerreviewed
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-018-33749-5
dc.identifier.journalScientific Reports
dc.repisalud.orgCNICCNIC::Unidades técnicas::Celómica
dc.repisalud.institucionCNIC
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD16/0025/0013es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PI16/01769es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CP14/00198es_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
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