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dc.contributor.authorGomez-Garay, Aranzazu
dc.contributor.authorLopez, Juan Antonio 
dc.contributor.authorCamafeita, Emilio 
dc.contributor.authorBueno, Maria Angeles
dc.contributor.authorPintos, Beatriz
dc.date.accessioned2018-11-21T08:36:53Z
dc.date.available2018-11-21T08:36:53Z
dc.date.issued2013-11-20
dc.identifier.citationJ Proteomics. 2013 Nov 20;93:314-25es_ES
dc.identifier.issn18743919es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6651
dc.description.abstractUNLABELLED: Quercus suber L. is a forest tree with remarkable ecological, social and economic value in the southern Europe ecosystems. To circumvent the difficulties of breeding such long-lived species like Q. suber in a conventional fashion, clonal propagation of Q. suber elite trees can be carried out, although this process is sometimes unsuccessful. To help decipher the complex program underlying the development of Q. suber somatic embryos from the first early stage until maturity, a proteomic approach based on DIGE and MALDI-MS has been envisaged. Results highlighted several key processes involved in the three developmental stages (proliferative, cotyledonary and mature) of Q. suber somatic embryogenesis studied. Results show that the proliferation stage is characterized by fermentation as an alternative energy source at the first steps of somatic embryo development, as well as by up-regulation of proteins involved in cell division. In this stage reactive oxygen species play a role in proliferation, while other proteins like CAD and PR5 seem to be implied in embryonic competence. In the transition to the cotyledonary stage diverse ROS detoxification enzymes are activated and reserve products (mainly carbohydrates and proteins) are accumulated, whereas energy production is increased probably to participate in the synthesis of primary metabolites such as amino acids and fatty acids. Finally, in the mature stage ethylene accumulation regulates embryo development. BIOLOGICAL SIGNIFICANCE: Quercus suber L. is a forest tree with remarkable ecological, social and economic value in the southern Europe ecosystems. To circumvent the difficulties of breeding such long-lived species like Q. suber in a conventional fashion, clonal propagation of Q. suber elite trees can be carried out, although this process is sometimes unsuccessful. To help decipher the complex program underlying the development of Q. suber somatic embryos from the first early stage until maturity, in deep studies become necessary. This article is part of a Special Issue entitled: Translational Plant Proteomics.es_ES
dc.description.sponsorshipThe Centro Nacional de Investigaciones Cardiovasculares is supported by the Spanish Ministerio de Economía y Competitividad and the Fundación Pro-CNIC.
dc.language.isoenges_ES
dc.relation.isversionofPostprintes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject5-Methyltetrahydropteroyltriglutamate–homocysteine methyltransferasees_ES
dc.subjectAAes_ES
dc.subjectAFRes_ES
dc.subjectAKRes_ES
dc.subjectAldo keto reductasees_ES
dc.subjectAscorbate free radicales_ES
dc.subjectAscorbic acides_ES
dc.subjectCADes_ES
dc.subjectCSEes_ES
dc.subjectCinnamyl alcohol dehydrogenasees_ES
dc.subjectCotyledonar somatic embryoses_ES
dc.subjectDHAes_ES
dc.subjectDIGEes_ES
dc.subjectDehydroascorbate (synonym: dehydroascorbic acid)es_ES
dc.subjectGAPDHes_ES
dc.subjectGlyceraldehyde-3-phosphate dehydrogenasees_ES
dc.subjectHeat shock proteines_ES
dc.subjectHspes_ES
dc.subjectLEAes_ES
dc.subjectLate embryogenesis abundantes_ES
dc.subjectMALDIes_ES
dc.subjectMSEes_ES
dc.subjectMTHPes_ES
dc.subjectMass spectrometryes_ES
dc.subjectMature somatic embryoses_ES
dc.subjectPCAes_ES
dc.subjectPR proteines_ES
dc.subjectPSEes_ES
dc.subjectPTMes_ES
dc.subjectPathogenesis-related proteines_ES
dc.subjectPost-translational modificationes_ES
dc.subjectPrincipal components analysises_ES
dc.subjectProliferating somatic embryoses_ES
dc.subjectProteomicses_ES
dc.subjectQuercuses_ES
dc.subjectROSes_ES
dc.subjectReactive oxygen specieses_ES
dc.subjectSODes_ES
dc.subjectSomatic embryogenesises_ES
dc.subjectSuperoxide dismutasees_ES
dc.subject.meshCloning, Organism es_ES
dc.subject.meshPlant Somatic Embryogenesis Techniques es_ES
dc.subject.meshProteome es_ES
dc.subject.meshQuercus es_ES
dc.subject.meshSeeds es_ES
dc.subject.meshUp-Regulation es_ES
dc.subject.meshProteomics es_ES
dc.titleProteomic perspective of Quercus suber somatic embryogenesises_ES
dc.typeArtículoes_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID23770300es_ES
dc.format.volume93es_ES
dc.format.page314-25es_ES
dc.identifier.doi10.1016/j.jprot.2013.06.006es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.contributor.funderFundación ProCNIC
dc.description.peerreviewedes_ES
dc.identifier.e-issn1876-7737es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.jprot.2013.06.006
dc.identifier.journalJournal of proteomicses_ES
dc.repisalud.orgCNICCNIC::Unidades técnicas::Proteómica / Metabolómicaes_ES
dc.repisalud.institucionCNICes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional