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dc.contributor.author | Isern, Joan | |
dc.contributor.author | Martín-Antonio, Beatriz | |
dc.contributor.author | Ghazanfari, Roshanak | |
dc.contributor.author | Martin, Ana M. | |
dc.contributor.author | Lopez, Juan Antonio | |
dc.contributor.author | Del Toro, Raquel | |
dc.contributor.author | Sanchez-Aguilera, Abel | |
dc.contributor.author | Arranz, Lorena | |
dc.contributor.author | Martin-Perez, Daniel | |
dc.contributor.author | Suárez-Lledó, María | |
dc.contributor.author | Marín, Pedro | |
dc.contributor.author | Van Pel, Melissa | |
dc.contributor.author | Fibbe, Willem E | |
dc.contributor.author | Vazquez, Jesus | |
dc.contributor.author | Scheding, Stefan | |
dc.contributor.author | Urbano-Ispizúa, Álvaro | |
dc.contributor.author | Mendez-Ferrer, Simon | |
dc.date.accessioned | 2018-11-20T13:45:55Z | |
dc.date.available | 2018-11-20T13:45:55Z | |
dc.date.issued | 2013-05-30 | |
dc.identifier.citation | Cell Rep. 2013 May 30;3(5):1714-24 | es_ES |
dc.identifier.issn | 22111247 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/6643 | |
dc.description.abstract | Strategies for expanding hematopoietic stem cells (HSCs) include coculture with cells that recapitulate their natural microenvironment, such as bone marrow stromal stem/progenitor cells (BMSCs). Plastic-adherent BMSCs may be insufficient to preserve primitive HSCs. Here, we describe a method of isolating and culturing human BMSCs as nonadherent mesenchymal spheres. Human mesenspheres were derived from CD45- CD31- CD71- CD146+ CD105+ nestin+ cells but could also be simply grown from fetal and adult BM CD45--enriched cells. Human mesenspheres robustly differentiated into mesenchymal lineages. In culture conditions where they displayed a relatively undifferentiated phenotype, with decreased adherence to plastic and increased self-renewal, they promoted enhanced expansion of cord blood CD34+ cells through secreted soluble factors. Expanded HSCs were serially transplantable in immunodeficient mice and significantly increased long-term human hematopoietic engraftment. These results pave the way for culture techniques that preserve the self-renewal of human BMSCs and their ability to support functional HSCs. | es_ES |
dc.language.iso | eng | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Antigens, CD | es_ES |
dc.subject.mesh | Bone Marrow Cells | es_ES |
dc.subject.mesh | Cell Differentiation | es_ES |
dc.subject.mesh | Cell Lineage | es_ES |
dc.subject.mesh | Cells, Cultured | es_ES |
dc.subject.mesh | Coculture Techniques | es_ES |
dc.subject.mesh | Fetal Blood | es_ES |
dc.subject.mesh | Hematopoietic Stem Cells | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Mesenchymal Stromal Cells | es_ES |
dc.subject.mesh | Mice | es_ES |
dc.subject.mesh | Mice, Inbred NOD | es_ES |
dc.subject.mesh | Nestin | es_ES |
dc.title | Self-renewing human bone marrow mesenspheres promote hematopoietic stem cell expansion | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.identifier.pubmedID | 23623496 | es_ES |
dc.format.volume | 3 | es_ES |
dc.format.number | 5 | es_ES |
dc.format.page | 1714-24 | es_ES |
dc.identifier.doi | 10.1016/j.celrep.2013.03.041 | es_ES |
dc.contributor.funder | Centro Nacional de Investigaciones Cardiovasculares Carlos III (España) | |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Unión Europea. Comisión Europea | |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Regional Government of Andalusia (España) | |
dc.contributor.funder | Swedish Research Council | |
dc.contributor.funder | Swedish Childhood Cancer Foundation | |
dc.contributor.funder | Comunidad de Madrid (España) | |
dc.contributor.funder | Ministerio de Educación (España) | |
dc.contributor.funder | European Hematology Association | |
dc.contributor.funder | Howard Hughes Medical Institute | |
dc.contributor.funder | Swedish Cancer Foundation | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2211-1247 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.celrep.2013.03.041 | |
dc.identifier.journal | Cell reports | es_ES |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Proteómica / Metabolómica | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Proteómica cardiovascular | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Antiguos CNIC | |
dc.repisalud.institucion | CNIC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/294262/EU | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/294096/EU | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2011-30308 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RYC-2011-09209 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RYC-2011-09726 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RYC-2009-04703 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/BIO2009-07990 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RD06/0014/0030 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/PI11/01090 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RD06/0020/0012 | es_ES |
dc.rights.accessRights | open access | es_ES |