dc.contributor.author | Erami, Roghayeh Sadeghi | |
dc.contributor.author | Ovejero, Karina | |
dc.contributor.author | Meghdadi, Soraia | |
dc.contributor.author | Filice, Marco | |
dc.contributor.author | Amirnasr, Mehdi | |
dc.contributor.author | Rodriguez-Dieguez, Antonio | |
dc.contributor.author | De La Orden, Maria Ulagares | |
dc.contributor.author | Gomez-Ruiz, Santiago | |
dc.date.accessioned | 2018-11-05T11:58:22Z | |
dc.date.available | 2018-11-05T11:58:22Z | |
dc.date.issued | 2018 | |
dc.identifier | ISI:000436505200079 | |
dc.identifier.citation | Nanomaterials (Basel). 2018; 8(6):434 | |
dc.identifier.issn | 2079-4991 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/6581 | |
dc.description.abstract | Functionalized magnetite nanoparticles (FMNPs) and functionalized mesoporous silica nanoparticles (FMSNs) were synthesized by the conjugation of magnetite and mesoporous silica with the small and fluorogenic benzothiazole ligand, that is, 2(2-hydroxyphenyl)benzothiazole (hpbtz). The synthesized fluorescent nanoparticles were characterized by FTIR, XRD, XRF, C-13 CP MAS NMR, BET, and TEM. The photophysical behavior of FMNPs and FMSNs in ethanol was studied using fluorescence spectroscopy. The modification of magnetite and silica scaffolds with the highly fluorescent benzothiazole ligand enabled the nanoparticles to be used as selective and sensitive optical probes for zinc ion detection. Moreover, the presence of hpbtz in FMNPs and FMSNs induced efficient cell viability and zinc ion uptake, with desirable signaling in the normal human kidney epithelial (Hek293) cell line. The significant viability of FMNPs and FMSNs (80\% and 92\%, respectively) indicates a potential applicability of these nanoparticles as in vitro imaging agents. The calculated limit of detections (LODs) were found to be 2.53 x 10(-6) and 2.55 x 10(-6) M for Fe3O4-H@hpbtz and MSN-Et3N-IPTMS-hpbtz-f1, respectively. FMSNs showed more pronounced zinc signaling relative to FMNPs, as a result of the more efficient penetration into the cells. | |
dc.description.sponsorship | This research was funded by several sources. The URJC authors thank the financial support of the Ministerio de Economia y Competitividad and FEDER (Grants nos. CTQ2015-66164-R and CTQ2017-90802-REDT) and Universidad Rey Juan Carlos-Banco de Santander for supporting our excellence group QUINANOAP. The partial support of this work by the Isfahan University of Technology Research Council (grant number 500/95/24305 and the Iran National Science Foundation through INSF grant number 95828071 is also acknowledged. The CNIC is supported by the Spanish Ministerio de Ciencia, Innovacion y Universidades and the Pro-CNIC Foundation and is a Severo Ochoa Center of Excellence (SEV-2015-0505). M.F. would like to thank MEyC for the research grant no. SAF2014-59118-JIN, co-funded by Fondo Europeo de Desarrollo Regional (FEDER) and COST Action CA1520: `European Network on NMR Relaxometry-EURELAX'. M.F. would also like to thank the Community of Madrid for research contract num. 2017-T1/BIO-4992 ('Atraccion de Talento' Action) cofunded by Universidad Complutense de Madrid. | |
dc.language.iso | eng | |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | |
dc.type.hasVersion | VoR | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Nanomaterials | |
dc.subject | Mesoporous silica | |
dc.subject | Magnetite | |
dc.subject | Live cell imaging | |
dc.subject | Zn2+ detection | |
dc.subject | Zn2+ sensors | |
dc.subject | COMBINED IONOPHORE/FLUOROPHORE MOIETIES | |
dc.subject | FLUORESCENT-PROBE | |
dc.subject | OXIDATIVE STRESS | |
dc.subject | AQUEOUS-SOLUTION | |
dc.subject | LIVING CELLS | |
dc.subject | ZN2+ ION | |
dc.subject | NANOPARTICLES | |
dc.subject | COMPLEXES | |
dc.subject | SENSOR | |
dc.subject | BENZOTHIAZOLE | |
dc.title | Applications of Nanomaterials Based on Magnetite and Mesoporous Silica on the Selective Detection of Zinc Ion in Live Cell Imaging | |
dc.type | journal article | |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 29903996 | |
dc.format.volume | 8 | |
dc.identifier.doi | 10.3390/nano8060434 | |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | |
dc.contributor.funder | King Juan Carlos University (España) | |
dc.contributor.funder | Isfahan University of Technology (Irán) | |
dc.contributor.funder | Iran National Science Foundation | |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | |
dc.contributor.funder | Fundación ProCNIC | |
dc.contributor.funder | Complutense University of Madrid (España) | |
dc.contributor.funder | Comunidad de Madrid (España) | |
dc.description.peerreviewed | Sí | |
dc.relation.publisherversion | https://doi.org/10.3390/nano8060434 | |
dc.identifier.journal | Nanomaterials | |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas | |
dc.repisalud.institucion | CNIC | |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2014-59118-JIN | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/CTQ2015-66164-R | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/CTQ2017-90802-REDT | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SEV-2015-0505 | es_ES |
dc.rights.accessRights | open access | es_ES |