Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/6521
Título
Chemokines induce axon outgrowth downstream of Hepatocyte Growth Factor and TCF/beta-catenin signaling
Autor(es)
Bhardwaj, Deepshikha | Nager, Mireia | Camats, Judith | David, Monica | Benguria, Alberto CNIC | Dopazo, Ana CNIC | Canti, Carles | Herreros, Judit
Fecha de publicación
2013
Cita
Front Cell Neurosci. 2013; 7:52
Idioma
Inglés
Tipo de documento
journal article
Resumen
Axon morphogenesis is a complex process regulated by a variety of secreted molecules, including morphogens and growth factors, resulting in the establishment of the neuronal circuitry. Our previous work demonstrated that growth factors [Neurotrophins (NT) and Hepatocyte Growth Factor (HGF)] signal through beta-catenin during axon morphogenesis. HGF signaling promotes axon outgrowth and branching by inducing beta-catenin phosphorylation at Y142 and transcriptional regulation of T-Cell Factor (TCF) target genes. Here, we asked which genes are regulated by HGF signaling during axon morphogenesis. An array screening indicated that HGF signaling elevates the expression of chemokines of the CC and CXC families. In line with this, CCL7, CCL20, and CXCL2 significantly increase axon outgrowth in hippocampal neurons. Experiments using blocking antibodies and chemokine receptor antagonists demonstrate that chemokines act downstream of HGF signaling during axon morphogenesis. In addition, qPCR data demonstrates that CXCL2 and CCL5 expression is stimulated by HGF through Met/b-catenin/TCF pathway. These results identify CC family members and CXCL2 chemokines as novel regulators of axon morphogenesis downstream of HGF signaling.
Palabras clave
Beta-catenin | Axon | Neurite outgrowth | Chemokine | Hippocampal neurons | Hepatocyte growth factor | CELL-DERIVED FACTOR-1 | BETA-CATENIN | INTERNEURON MIGRATION | HIPPOCAMPAL-NEURONS | NEURITE OUTGROWTH | SYNAPSE FORMATION | NERVOUS-SYSTEM | DENTATE GYRUS | RECEPTORS | CXCR4
Versión en línea
DOI
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