Mostrar el registro sencillo del ítem
dc.contributor.author | Alvaro-Blanco, Josue | |
dc.contributor.author | Urso, Katia | |
dc.contributor.author | Chiodo, Yuri | |
dc.contributor.author | Martin-Cortazar, Carla | |
dc.contributor.author | Kourani, Omar | |
dc.contributor.author | Gomez-del Arco, Pablo | |
dc.contributor.author | Rodriguez-Martinez, Maria | |
dc.contributor.author | Calonge, Esther | |
dc.contributor.author | Alcamí, José | |
dc.contributor.author | Redondo, Juan Miguel | |
dc.contributor.author | Iglesias, Teresa | |
dc.contributor.author | Campanero, Miguel R. | |
dc.date.accessioned | 2018-10-19T08:00:43Z | |
dc.date.available | 2018-10-19T08:00:43Z | |
dc.date.issued | 2017 | |
dc.identifier | ISI:000412008000017 | |
dc.identifier.citation | Nucleic Acids Res. 2017; 45(17):9960-9975 | |
dc.identifier.issn | 0305-1048 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/6497 | |
dc.description.abstract | Most E2F-binding sites repress transcription through the recruitment of Retinoblastoma (RB) family members until the end of the G1 cell-cycle phase. Although the MYB promoter contains an E2F-binding site, its transcription is activated shortly after the exit from quiescence, before RB family members inactivation, by unknown mechanisms. We had previously uncovered a nuclear factor distinct from E2F, Mybsp, whose DNA-binding site overlapped the E2F element and had hypothesized that this factor might overcome the transcriptional repression of MYB by E2F-RB family members. We have purified Myb-sp and discovered that Myc-associated zinc finger proteins (MAZ) are major components. We show that various MAZ isoforms are present in Myb-sp and activate transcription via the MYB-E2F element. Moreover, while forced RB or p130 expression repressed the activity of a luciferase reporter driven by the MYB-E2F element, co-expression of MAZ proteins not only reverted repression, but also activated transcription. Finally, we show that MAZ binds the MYB promoter in vivo, that its binding site is critical for MYB transactivation, and that MAZ knockdown inhibits MYB expression during the exit from quiescence. Together, these data indicate that MAZ is essential to bypass MYB promoter repression by RB family members and to induce MYB expression. | |
dc.description.sponsorship | Spanish Ministerio de Economia, Industria y Competitividad [SAF2013-45258P to M.R.C., SAF2014-52737P to T.I.]; Instituto de Salud Carlos III [FIS PI12/0056 to J.A., CIBERNED to T.I.]; Spanish AIDS Research Network [RD16CIII/0002/0001 to J.A.]; FEDER funds (in part); Spanish National Center for Biotechnology [PT13/0001]. Funding for open access charge: Spanish Ministerio de Economia, Industria y Competitividad [SAF2013-45258P]. | |
dc.language.iso | eng | |
dc.publisher | Oxford University Press | |
dc.type.hasVersion | VoR | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | ZINC-FINGER PROTEIN | |
dc.subject | CELL-CYCLE CONTROL | |
dc.subject | C-MYB | |
dc.subject | TRANSCRIPTION FACTORS | |
dc.subject | BINDING-SITES | |
dc.subject | NEGATIVE REGULATION | |
dc.subject | SIGNALING PATHWAY | |
dc.subject | GENE-EXPRESSION | |
dc.subject | SPLICE VARIANT | |
dc.subject | RECEPTOR GENE | |
dc.title | MAZ induces MYB expression during the exit from quiescence via the E2F site in the MYB promoter | |
dc.type | journal article | |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 28973440 | |
dc.format.volume | 45 | |
dc.format.page | 9960-9975 | |
dc.identifier.doi | 10.1093/nar/gkx641 | |
dc.contributor.funder | Ministerio de Economía, Industria y Competitividad (España) | |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Centro de Investigación Biomedica en Red - CIBER | |
dc.contributor.funder | Red de Investigación Cooperativa en Investigación en Sida (España) | |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | |
dc.description.peerreviewed | Sí | |
dc.identifier.e-issn | 1362-4962 | |
dc.relation.publisherversion | https://doi.org/10.1093/nar/gkx641 | |
dc.identifier.journal | Nucleic Acids Research | |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Regulación Génica en Remodelado Vascular e Inflamación | |
dc.repisalud.institucion | CNIC | |
dc.relation.projectID | MINECO/ICTI2013-2016/SAF2013-45258P | es_ES |
dc.relation.projectID | MINECO/ICTI2013-2016/SAF2014-52737P | es_ES |
dc.rights.accessRights | open access | es_ES |