dc.contributor.author | Alvaro-Blanco, Josue | |
dc.contributor.author | Urso, Katia | |
dc.contributor.author | Chiodo, Yuri | |
dc.contributor.author | Martin-Cortazar, Carla | |
dc.contributor.author | Kourani, Omar | |
dc.contributor.author | Gomez-del Arco, Pablo | |
dc.contributor.author | Rodriguez-Martinez, Maria | |
dc.contributor.author | Calonge, Esther | |
dc.contributor.author | Alcamí, José | |
dc.contributor.author | Redondo, Juan Miguel | |
dc.contributor.author | Iglesias, Teresa | |
dc.contributor.author | Campanero, Miguel R. | |
dc.date.accessioned | 2018-10-19T08:00:43Z | |
dc.date.available | 2018-10-19T08:00:43Z | |
dc.date.issued | 2017 | |
dc.identifier | ISI:000412008000017 | |
dc.identifier.citation | Nucleic Acids Res. 2017; 45(17):9960-9975 | |
dc.identifier.issn | 0305-1048 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/6497 | |
dc.description.abstract | Most E2F-binding sites repress transcription through the recruitment of
Retinoblastoma (RB) family members until the end of the G1 cell-cycle
phase. Although the MYB promoter contains an E2F-binding site, its
transcription is activated shortly after the exit from quiescence,
before RB family members inactivation, by unknown mechanisms. We had
previously uncovered a nuclear factor distinct from E2F, Mybsp, whose
DNA-binding site overlapped the E2F element and had hypothesized that
this factor might overcome the transcriptional repression of MYB by
E2F-RB family members. We have purified Myb-sp and discovered that
Myc-associated zinc finger proteins (MAZ) are major components. We show
that various MAZ isoforms are present in Myb-sp and activate
transcription via the MYB-E2F element. Moreover, while forced RB or p130
expression repressed the activity of a luciferase reporter driven by the
MYB-E2F element, co-expression of MAZ proteins not only reverted
repression, but also activated transcription. Finally, we show that MAZ
binds the MYB promoter in vivo, that its binding site is critical for
MYB transactivation, and that MAZ knockdown inhibits MYB expression
during the exit from quiescence. Together, these data indicate that MAZ
is essential to bypass MYB promoter repression by RB family members and
to induce MYB expression. | |
dc.description.sponsorship | Spanish Ministerio de Economia, Industria y Competitividad
[SAF2013-45258P to M.R.C., SAF2014-52737P to T.I.]; Instituto de Salud
Carlos III [FIS PI12/0056 to J.A., CIBERNED to T.I.]; Spanish AIDS
Research Network [RD16CIII/0002/0001 to J.A.]; FEDER funds (in part);
Spanish National Center for Biotechnology [PT13/0001]. Funding for
open access charge: Spanish Ministerio de Economia, Industria y
Competitividad [SAF2013-45258P]. | |
dc.language.iso | eng | |
dc.publisher | Oxford University Press | |
dc.type.hasVersion | VoR | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | ZINC-FINGER PROTEIN | |
dc.subject | CELL-CYCLE CONTROL | |
dc.subject | C-MYB | |
dc.subject | TRANSCRIPTION FACTORS | |
dc.subject | BINDING-SITES | |
dc.subject | NEGATIVE REGULATION | |
dc.subject | SIGNALING PATHWAY | |
dc.subject | GENE-EXPRESSION | |
dc.subject | SPLICE VARIANT | |
dc.subject | RECEPTOR GENE | |
dc.title | MAZ induces MYB expression during the exit from quiescence via the E2F site in the MYB promoter | |
dc.type | journal article | |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 28973440 | |
dc.format.volume | 45 | |
dc.format.page | 9960-9975 | |
dc.identifier.doi | 10.1093/nar/gkx641 | |
dc.contributor.funder | Ministerio de Economía, Industria y Competitividad (España) | |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Centro de Investigación Biomedica en Red - CIBER | |
dc.contributor.funder | Red de Investigación Cooperativa en Investigación en Sida (España) | |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | |
dc.description.peerreviewed | Sí | |
dc.identifier.e-issn | 1362-4962 | |
dc.relation.publisherversion | https://doi.org/10.1093/nar/gkx641 | |
dc.identifier.journal | Nucleic Acids Research | |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Regulación Génica en Remodelado Vascular e Inflamación | |
dc.repisalud.institucion | CNIC | |
dc.relation.projectID | MINECO/ICTI2013-2016/SAF2013-45258P | es_ES |
dc.relation.projectID | MINECO/ICTI2013-2016/SAF2014-52737P | es_ES |
dc.rights.accessRights | open access | es_ES |