Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/5411
Título
Altered Metabolic and Stemness Capacity of Adipose Tissue-Derived Stem
Cells from Obese Mouse and Human
Autor(es)
Perez, Laura M. CNIC | Bernal, Aurora CNIC | de Lucas, Beatriz CNIC | San Martin, Nuria CNIC | Mastrangelo, Annalaura CNIC | Garcia, Antonia | Barbas, Coral | Galvez, Beatriz G. CNIC
Fecha de publicación
2015
Cita
PLoS One. 2015; 10(4):e0123397
Idioma
Inglés
Tipo de documento
journal article
Resumen
Adipose stem cells (ASCs) are an appealing source of cells for
therapeutic intervention; however, the environment from which ASCs are
isolated may impact their usefulness. Using a range of functional
assays, we have evaluated whether ASCs isolated from an obese
environment are comparable to cells from non-obese adipose tissue.
Results showed that ASCs isolated from obese tissue have a reduced
proliferative ability and a loss of viability together with changes in
telomerase activity and DNA telomere length, suggesting a decreased
self-renewal capacity. Metabolic analysis demonstrated that
mitochondrial content and function was impaired in obese-derived ASCs
resulting in changes in favored oxidative substrates. These findings
highlight the impact of obesity on adult stem properties. Hence, caution
should be exercised when considering the source of ASCs for cellular
therapies since their therapeutic potential may be impaired.
Palabras clave
MITOCHONDRIAL DYSFUNCTION | INSULIN-RESISTANCE | ENERGY-METABOLISM | PROGENITOR CELLS | SELF-RENEWAL | EXPRESSION | OCT4 | RESPIRATION | ADIPOCYTES | GLYCOLYSIS
Versión en línea
DOI
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