Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/5396
Título
Signature of microRNA expression during osteogenic differentiation of
bone marrow MSCs reveals a putative role of miR-335-5p in osteoarthritis
Autor(es)
Fecha de publicación
2015
Cita
BMC Musculoskelet Disord. 2015; 16:182
Idioma
Inglés
Tipo de documento
journal article
Resumen
Background: The aim of this study was to evaluate, the existence of a
signature of differentially expressed microRNAs (miRNAs) during
osteogenic differentiation of bone marrow MSCs from OA and healthy
donors and to describe their possible implication in joint regeneration
through modulation of molecular mechanisms involved in homeostatic
control in OA pathophysiology.
Methods: Following phenotypic assessment of BM-MSCs obtained from OA
diagnosed patients (n = 10) and non-OA (n = 10), total small RNA was
isolated after osteogenic induction for 1, 10 and 21 days, miRNA
profiles were generated using a commercial expression array of 754
well-characterized miRNAs. MiRNAs, with consistent differential
expression were selected for further validation by quantitative
reverse-transcription polymerase chain reaction (qRT-PCR) analysis.
Results: A total of 246 miRNAs were differentially expressed (fold
change >=+/- 2, P <= 0.05) between OA and non-OA BM-MSC samples; these
miRNAs showed variable interactions depending on the cell and
differentiation status. Two miRNAs, hsa-miR-210 and hsa-miR-335-5p out
of 21 used for validation showed a significant downregulated expression
during induced osteogenesis. In particular hsa-miR-335-5p, a critical
regulator in bone homeostasis, was further studied. hsa-miR-335-5p
downregulation in OA-MSCs, as well as their host coding gene, MEST, were
also assessed.
Conclusions: To our knowledge, this study represents the most
comprehensive assessment to date of miRNA expression profiling in
BM-MSCs from OA patients and their role during osteogenic
differentiation. We describe the existence of a correlation between
miR-335-5p expression and OA indicating the putative role of this miRNA
in OA features. These findings, may contribute to our understanding of
the molecular mechanisms involved in MSCs mediated homeostatic control
in OA pathophysiology that could be applicable in future therapeutic
approaches.
Palabras clave
MESENCHYMAL STEM-CELLS | GENE-EXPRESSION | BETA-CATENIN | ENDOCHONDRAL
OSSIFICATION | ARTICULAR-CARTILAGE | SIGNALING PATHWAY | WNT | CHONDROGENESIS | PATHOGENESIS | DEGRADATION
Versión en línea
DOI
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