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dc.contributor.authordel Olmo, Ivan
dc.contributor.authorLopez, Juan Antonio 
dc.contributor.authorVazquez, Jesus 
dc.contributor.authorRaynaud, Cecile
dc.contributor.authorPineiro, Manuel
dc.contributor.authorJarillo, Jose A.
dc.date.accessioned2017-10-30T13:15:41Z
dc.date.available2017-10-30T13:15:41Z
dc.date.issued2016
dc.identifierISI:000381210900015
dc.identifier.citationNucleic Acids Res. 2016; 44(12):5597-614
dc.identifier.issn0305-1048
dc.identifier.urihttp://hdl.handle.net/20.500.12105/5211
dc.description.abstractArabidopsis ESD7 locus encodes the catalytic subunit of the DNA Pol I mu involved in the synthesis of the DNA leading strand and is essential for embryo viability. The hypomorphic allele esd7-1 is viable but displays a number of pleiotropic phenotypic alterations including an acceleration of flowering time. Furthermore, Pol I mu is involved in the epigenetic silencing of the floral integrator genes FT and SOC1, but the molecular nature of the transcriptional gene silencing mechanisms involved remains elusive. Here we reveal that ESD7 interacts with components of the PRC2 such as CLF, EMF2 and MSI1, and that mutations in ESD7 cause a decrease in the levels of the H3K27me3 mark present in the chromatin of FT and SOC1. We also demonstrate that a domain of the C-terminal region of ESD7 mediates the binding to the different PRC2 components and this interaction is necessary for the proper recruitment of PRC2 to FT and SOC1 chromatin. We unveil the existence of interplay between the DNA replication machinery and the PcG complexes in epigenetic transcriptional silencing. These observations provide an insight into the mechanisms ensuring that the epigenetic code at pivotal loci in developmental control is faithfully transmitted to the progeny of eukaryotic cells.
dc.description.sponsorshipSpanish Ministerio de Economia y Competitividad MINECO [BIO2010-15589 to M.P., J.A.J.]; Spanish Ministerio de Ciencia e Innovacion [BIO2013-43098R to M.P., J.A.J.]. Funding for open access charge: MINECO [BIO2013-43098R].
dc.language.isoeng
dc.publisherOxford University Press 
dc.type.hasVersionVoR
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectFLOWERING-LOCUS-T
dc.subjectCHROMATIN ASSEMBLY FACTOR-1
dc.subjectHISTONE H3
dc.subjectHETEROCHROMATIN PROTEIN-1
dc.subjectCATALYTIC SUBUNIT
dc.subjectSHADOW DOMAIN
dc.subjectSALT STRESS
dc.subjectCELL-CYCLE
dc.subjectREPLICATION
dc.subjectTHALIANA
dc.titleArabidopsis DNA polymerase epsilon recruits components of Polycomb repressor complex to mediate epigenetic gene silencing
dc.typejournal article
dc.rights.licenseAtribución-NoComercial 4.0 Internacional*
dc.identifier.pubmedID26980282
dc.format.volume44
dc.format.page5597-5614
dc.identifier.doi10.1093/nar/gkw156
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.contributor.funderMinisterio de Ciencia e Innovación (España) 
dc.description.peerreviewed
dc.identifier.e-issn1362-4962
dc.relation.publisherversionhttps://doi.org/10.1093/nar/gkw156
dc.identifier.journalNucleic Acids Research
dc.repisalud.orgCNICCNIC::Unidades técnicas::Proteómica / Metabolómica
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovascular
dc.repisalud.institucionCNIC
dc.relation.projectIDMINECO/ICTI2013-2016/BIO2013-43098Res_ES
dc.rights.accessRightsopen accesses_ES


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Atribución-NoComercial 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución-NoComercial 4.0 Internacional