Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/5170
Título
Quantitative HDL Proteomics Identifies Peroxiredoxin-6 as a Biomarker of Human Abdominal Aortic Aneurysm
Autor(es)
Burillo, Elena | Jorge, Inmaculada CNIC | Martinez-Lopez, Diego | Camafeita, Emilio CNIC | Miguel Blanco-Colio, Luis | Trevisan-Herraz, Marco CNIC | Ezkurdia, Iakes CNIC | Egido, Jesus | Michel, Jean-Baptiste | Meilhac, Olivier | Vazquez, Jesus CNIC | Luis Martin-Ventura, Jose
Fecha de publicación
2016
Cita
Sci Rep. 2016; 6:38477
Idioma
Inglés
Tipo de documento
journal article
Resumen
High-density lipoproteins (HDLs) are complex protein and lipid assemblies whose composition is known to change in diverse pathological situations. Analysis of the HDL proteome can thus provide insight into the main mechanisms underlying abdominal aortic aneurysm (AAA) and potentially detect novel systemic biomarkers. We performed a multiplexed quantitative proteomics analysis of HDLs isolated from plasma of AAA patients (N = 14) and control study participants (N = 7). Validation was performed by western-blot (HDL), immunohistochemistry (tissue), and ELISA (plasma). HDL from AAA patients showed elevated expression of peroxiredoxin-6 (PRDX6), HLA class I histocompatibility antigen (HLA-I), retinol-binding protein 4, and paraoxonase/arylesterase 1 (PON1), whereas alpha-2 macroglobulin and C4b-binding protein were decreased. The main pathways associated with HDL alterations in AAA were oxidative stress and immune-inflammatory responses. In AAA tissue, PRDX6 colocalized with neutrophils, vascular smooth muscle cells, and lipid oxidation. Moreover, plasma PRDX6 was higher in AAA (N = 47) than in controls (N = 27), reflecting increased systemic oxidative stress. Finally, a positive correlation was recorded between PRDX6 and AAA diameter. The analysis of the HDL proteome demonstrates that redox imbalance is a major mechanism in AAA, identifying the antioxidant PRDX6 as a novel systemic biomarker of AAA.
Palabras clave
HIGH-DENSITY-LIPOPROTEINS | NADPH OXIDASE ACTIVITY | COMPLEMENT ACTIVATION | OXIDATIVE STRESS | SMOOTH-MUSCLE | ATHEROSCLEROSIS | DISCOVERY | RUPTURE | CELLS
Versión en línea
DOI
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