dc.contributor.author | Pontes-Quero, Samuel | |
dc.contributor.author | Heredia, Luis | |
dc.contributor.author | Casquero-Garcia, Veronica | |
dc.contributor.author | Fernandez-Chacon, Macarena | |
dc.contributor.author | Luo, Wen | |
dc.contributor.author | Hermoso, Ana | |
dc.contributor.author | Bansal, Mayank | |
dc.contributor.author | Garcia-Gonzalez, Irene | |
dc.contributor.author | Sanchez-Munoz, Maria S. | |
dc.contributor.author | Perea, Juan R. | |
dc.contributor.author | Galiana-Simal, Adrian | |
dc.contributor.author | Rodriguez-Arabaolaza, Iker | |
dc.contributor.author | Del Olmo-Cabrera, Sergio | |
dc.contributor.author | Rocha, Susana | |
dc.contributor.author | Criado-Rodriguez, Luis M. | |
dc.contributor.author | Giovinazzo, Giovanna | |
dc.contributor.author | Benedito, Rui | |
dc.date.accessioned | 2017-10-20T10:23:09Z | |
dc.date.available | 2017-10-20T10:23:09Z | |
dc.date.issued | 2017 | |
dc.identifier | ISI:000407445700019 | |
dc.identifier.citation | Cell. 2017; 170(4):800-814 e18 | |
dc.identifier.issn | 0092-8674 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/5100 | |
dc.description.abstract | Improved methods for manipulating and analyzing gene function have provided a better understanding of how genes work during organ development and disease. Inducible functional genetic mosaics can be extraordinarily useful in the study of biological systems; however, this experimental approach is still rarely used in vertebrates. This is mainly due to technical difficulties in the assembly of large DNA constructs carrying multiple genes and regulatory elements and their targeting to the genome. In addition, mosaic phenotypic analysis, unlike classical single gene-function analysis, requires clear labeling and detection of multiple cell clones in the same tissue. Here, we describe several methods for the rapid generation of transgenic or gene-targeted mice and embryonic stem (ES) cell lines containing all the necessary elements for inducible, fluorescent, and functional genetic mosaic (ifgMosaic) analysis. This technology enables the interrogation of multiple and combinatorial gene function with high temporal and cellular resolution. | |
dc.description.sponsorship | This work was supported by grants to the PI R.B. from the Spanish Ministry of Economy, Industry and Competitiveness (SAF2013-44329-P, SAF2013-42359-ERC, and RYC-2013-13209) and European Research Council (ERC-2014-StG - 638028). S.P.-Q., M.F.-C., and I.G.-G. were supported by PhD fellowships from Fundacion La Caixa (CX-SO-2013-02, CX\_E-2015-01, and CX-SO-16-1, respectively). W.L. by a FP7-PEOPLE-2012-COFUND GA600396 postdoctoral contract. We thank Simon Bartlett for English editing, Ralf H. Adams for sharing the Cdh5(PAC)-CreERT2 mice, Jose Luis de La Pompa for comments throughout the project and for sharing the Tie2-Cre mice, Gonzalo Gancedo for the help with the mouse colony, Valeria Caiolfa for the help with the microscopy, and all the members of the CNIC gene targeting, transgenesis, cellomics, and microscopy units. The CNIC is supported by MEIC/MINECO and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence (SEV-2015-0505). | |
dc.language.iso | eng | |
dc.publisher | Cell Press | |
dc.type.hasVersion | VoR | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | CRE-LOXP | |
dc.subject | IN-VIVO | |
dc.subject | MOUSE | |
dc.subject | MICE | |
dc.subject | EXPRESSION | |
dc.subject | RECOMBINATION | |
dc.subject | SYSTEM | |
dc.title | Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis | |
dc.type | journal article | |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 28802047 | |
dc.format.volume | 170 | |
dc.format.page | 800+ | |
dc.identifier.doi | 10.1016/j.cell.2017.07.031 | |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Unión Europea. Comisión Europea. European Research Council (ERC) | |
dc.contributor.funder | Fundación La Caixa | |
dc.contributor.funder | Fundación ProCNIC | |
dc.description.peerreviewed | Sí | |
dc.identifier.e-issn | 1097-4172 | |
dc.relation.publisherversion | https://doi.org/10.1016/j.cell.2017.07.031 | |
dc.identifier.journal | Cell | |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Genética Funcional del Sistema de Fosforilación Oxidativa | |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Transgénesis | |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Células Pluripotentes | |
dc.repisalud.institucion | CNIC | |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2013-44329-P | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2013-42359-ERC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/RYC-2013-13209 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SEV-2015-0505 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/600396/EU | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/638028/EU | es_ES |
dc.rights.accessRights | open access | es_ES |