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dc.contributor.author | Lozano-Chamizo, Laura | |
dc.contributor.author | Márquez, Carlos | |
dc.contributor.author | Marciello, Marzia | |
dc.contributor.author | Galdon, José Carlos | |
dc.contributor.author | de la Fuente-Zapico, Elsa | |
dc.contributor.author | Martinez-Mazón, Paula | |
dc.contributor.author | Gonzalez-Rumayor, Víctor | |
dc.contributor.author | Filice, Marco | |
dc.contributor.author | Gamiz, Francisco | |
dc.date.accessioned | 2024-07-09T09:14:10Z | |
dc.date.available | 2024-07-09T09:14:10Z | |
dc.date.issued | 2024-04-15 | |
dc.identifier.citation | Biosens Bioelectron. 2024 Apr 15:250:116040. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/20278 | |
dc.description.abstract | The COVID-19 pandemic has taught us valuable lessons, especially the urgent need for a widespread, rapid and sensitive diagnostic tool. To this, the integration of bidimensional nanomaterials, particularly graphene, into point-of-care biomedical devices is a groundbreaking strategy able to potentially revolutionize the diagnostic landscape. Despite advancements in the fabrication of these biosensors, the relationship between their surface biofunctionalization and sensing performance remains unclear. Here, we demonstrate that the combination of careful sensor fabrication and its precise surface biofunctionalization is crucial for exalting the sensing performances of 2D biosensors. Specifically, we have biofunctionalized Graphene Field-Effect Transistor (GFET) sensors surface through different biochemical reactions to promote either random/heterogeneous or oriented/homogeneous immobilization of the Anti-SARS-CoV-2 spike protein antibody. Each strategy was thoroughly characterized by in-silico simulations, physicochemical and biochemical techniques and electrical characterization. Subsequently, both biosensors were tested in the label-free direct titration of SARS-CoV-2 virus in simulated clinical samples, avoiding sample preprocessing and within short timeframes. Remarkably, the oriented GFET biosensor exhibited significantly enhanced reproducibility and responsiveness, surpassing the detection sensitivity of conventional non-oriented GFET by more than twofold. This breakthrough not only involves direct implications for COVID-19 surveillance and next pandemic preparedness but also clarify an unexplored mechanistic dimension of biosensor research utilizing 2D-nanomaterials. | es_ES |
dc.description.sponsorship | L.L.C., M.M., V.G.R. and M.F. would like to thank Comunidad de Madrid for the predoctoral grant IND2020/BIO-17523. M.F. is grateful to Instituto de Salud Carlos III (ISCIII) for project No DTS20/00109 (AES20-ISCIII) and PI22/00789 (AES22-ISCIII). L.L.C., M.F. and M.M. acknowledge the support of Microscopy & Dynamic Imaging Unit of CNIC, Madrid, Spain. The Unit is part of the ReDiB-ICTS and has the support of FEDER, “Una manera de hacer Europa.” The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovacion ´ (MCIN) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (grant CEX 2020-001041-S funded by MICIN/AEI/10.13039/501100011033). The support of Spanish Ministerio de Ciencia e Innovacion ´ (MCIN) for funding the METALBIO Research Network (reference: RED 2022-134091-T) and the project CPP2022-009952 (MCIN/AEI /10.13039/501100011033, also funded by European Union NextGenerationEU/ PRTR) is acknowledged. M.M. and M.F. would like to thank Comunidad de Madrid for funding the REACT ANTICIPA-CM project. F.G. is grateful to Instituto de Salud Carlos III (ISCIII) for project No DTS20/00038, and Agencia Espanola ˜ de Investigacion ´ for research project PID 2021-128547OB-I00. The University of Granada group is also grateful to Junta de Andalucía for research projects P18-RT-4826, PYC-020-RE-023UGR, A-TIC-628- UGR20 (FEDER). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | COVID-19 | es_ES |
dc.subject.mesh | Biosensing Techniques | es_ES |
dc.subject.mesh | Graphite | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | SARS-CoV-2 | es_ES |
dc.subject.mesh | Pandemics | es_ES |
dc.subject.mesh | Reproducibility of Results | es_ES |
dc.title | High enhancement of sensitivity and reproducibility in label-free SARS-CoV-2 detection with graphene field-effect transistor sensors through precise surface biofunctionalization control. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 38290380 | es_ES |
dc.format.volume | 250 | es_ES |
dc.format.page | 116040 | es_ES |
dc.identifier.doi | 10.1016/j.bios.2024.116040 | es_ES |
dc.contributor.funder | Comunidad de Madrid (España) | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Fundación ProCNIC | es_ES |
dc.contributor.funder | Unión Europea. Comisión Europea. NextGenerationEU | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación (España) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1873-4235 | es_ES |
dc.relation.publisherversion | 10.1016/j.bios.2024.116040 | es_ES |
dc.identifier.journal | Biosensors & bioelectronics | es_ES |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Microscopía | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/IND2020/BIO-17523 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/DTS20/00109-AES20-ISCIII | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PI22/00789-AES22-ISCIII | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/MICIN/AEI/10.13039/501100011033/CEX 2020-001041-S | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/2022-134091-T | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/DTS20/00038 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PID-2021-128547OB-I00 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/P18-RT-4826 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PYC-020-RE-023UGR | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/A-TIC-628-UGR20 | es_ES |