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dc.contributor.author | Garcia-Gonzalez, Irene | |
dc.contributor.author | Rocha, Susana F | |
dc.contributor.author | Hamidi, Anahita | |
dc.contributor.author | Garcia-Ortega, Lourdes | |
dc.contributor.author | Regano, Alvaro | |
dc.contributor.author | Sanchez-Muñoz, Maria S | |
dc.contributor.author | Lytvyn, Mariya | |
dc.contributor.author | Garcia-Cabero, Aroa | |
dc.contributor.author | Roig-Soucase, Sergi | |
dc.contributor.author | Schoofs, Hans | |
dc.contributor.author | Castro, Marco | |
dc.contributor.author | Sabata, Helena | |
dc.contributor.author | Potente, Michael | |
dc.contributor.author | Graupera, Mariona | |
dc.contributor.author | Makinen, Taija | |
dc.contributor.author | Benedito, Rui | |
dc.date.accessioned | 2024-07-08T12:17:33Z | |
dc.date.available | 2024-07-08T12:17:33Z | |
dc.date.issued | 2024-06-08 | |
dc.identifier.citation | Nucleic Acids Res. 2024 Jun 8:gkae472. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/20205 | |
dc.description.abstract | Methods for modifying gene function at high spatiotemporal resolution in mice have revolutionized biomedical research, with Cre-loxP being the most widely used technology. However, the Cre-loxP technology has several drawbacks, including weak activity, leakiness, toxicity, and low reliability of existing Cre-reporters. This is mainly because different genes flanked by loxP sites (floxed) vary widely in their sensitivity to Cre-mediated recombination. Here, we report the generation, validation, and utility of iSuRe-HadCre, a new dual Cre-reporter and deleter mouse line that avoids these drawbacks. iSuRe-HadCre achieves this through a novel inducible dual-recombinase genetic cascade that ensures that cells expressing a fluorescent reporter had only transient Cre activity, that is nonetheless sufficient to effectively delete floxed genes. iSuRe-HadCre worked reliably in all cell types and for the 13 floxed genes tested. This new tool will enable the precise, efficient, and trustworthy analysis of gene function in entire mouse tissues or in single cells. | es_ES |
dc.description.sponsorship | This study was supported by grants to the PI R.B., from the Ministerio de Ciencia e Innovación (MCIN - PID2020- 120252RB-I00), Fundacion La Caixa (HR19-00120) and European Research Council (ERC-2020-COG- 101001814). M.P. laboratory was supported by the European Research Council (ERC) Consolidator grant (EMERGE-773047) and a Leducq Foundation grant. T.M laboratory was funded by the Knut and Alice Wallenberg Foundation (2018.0218) and Göran Gustafsson foundation. I.G-G was supported by a PhD fellowship from Fundación La Caixa (CX-SO-16-1). A.H was supported by a scholarship from the Swedish Society for Medical Research (SSMF). H.S. was supported by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 814316. A special thanks to ‘ReDIB ICTS infrastructure TRIMA@CNIC, Ministerio de Ciencia e Innovación (MCIN)’ for some of the CNIC microscopes used. The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación (MCIN) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (grant CEX2020-001041-S funded by MICIN/AEI/10.13039/501100011033). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | iSuRe-HadCre is an essential tool for effective conditional genetics. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 38850155 | es_ES |
dc.identifier.doi | 10.1093/nar/gkae472 | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Fundación La Caixa | es_ES |
dc.contributor.funder | Unión Europea. Comisión Europea. European Research Council (ERC) | es_ES |
dc.contributor.funder | Unión Europea. Comisión Europea. H2020 | es_ES |
dc.contributor.funder | Marie Curie | es_ES |
dc.contributor.funder | Fundación ProCNIC | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1362-4962 | es_ES |
dc.relation.publisherversion | 10.1093/nar/gkae472 | es_ES |
dc.identifier.journal | Nucleic acids research | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Genética Molecular de la Angiogénesis | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/ERC-2020-COG- 101001814 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/ERC/CoG/EMERGE-773047 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/CX-SO-16-1 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/814316 | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/MCIN-PID2020-120252RB-I00 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/HR19-00120 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/MICIN/AEI/10.13039/501100011033/CEX2020-001041-S | es_ES |