dc.contributor.author | Rodríguez, Diego | |
dc.contributor.author | Moscoso, Miguel | |
dc.contributor.author | Desco, Manuel | |
dc.contributor.author | Ripoll, Jorge | |
dc.contributor.author | Fernández, Roberto | |
dc.date.accessioned | 2024-07-03T09:54:53Z | |
dc.date.available | 2024-07-03T09:54:53Z | |
dc.date.issued | 2024-04-25 | |
dc.identifier.citation | Sci Rep. 2024 Apr 25;14(1):9536. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/19931 | |
dc.description.abstract | Neocaridina davidi, a small freshwater shrimp native to Asia, specifically China, Japan, Korea, and Vietnam, possesses remarkable resistance to poor water quality and offers various advantages over other invertebrate species to examine crucial issues in neuroscience and other related areas. These advantages include robustness, ease of maintenance, and transparency, making them useful for in vivo studies with optical imaging techniques. Despite its suitability for research purposes, particularly in the fields of imaging and fluorescent techniques, the lack of attention given to this species has resulted in the absence of a robust and replicable sedation protocol for immobilization and safe manipulation. Consequently, researchers face challenges in performing experimental procedures while minimizing harm to this specimen. In this study, we have developed and evaluated a simple sedation protocol specifically designed for Neocaridina davidi, assessing its effectiveness using light microscopy and image processing. | es_ES |
dc.description.sponsorship | RF acknowledges funding from Ministerio de Ciencia e Innovación of Spain (project PID2021-123124OB-I00).
JR and MM acknowledges funding from the Ministerio de Ciencia e Innovación (PID2020-115088RB-I00,
“BEHAVE-3D”). MD acknowledges funding from Instituto de Salud Carlos III through the project DTS22 /00030.
Tis project was co-funded by the European Union (ERDF, “A way to make Europe”), partially supported by
Comunidad de Madrid (S2017/BMD-3867 RENIM-CM), and co-fnanced by European Structural and Investment Fund. Te CNIC is supported by Instituto de Salud Carlos III (ISCIII), Ministerio de Ciencia e Innovación
(MCIN) and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV-2015-0505). Figures 1,
2, 3 and 4 were created with Biorender.com. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | Decapoda | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.title | Development and testing of a sedation protocol for Neocaridina davidi. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 38664522 | es_ES |
dc.format.volume | 14 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 9536 | es_ES |
dc.identifier.doi | 10.1038/s41598-024-60158-8 | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | es_ES |
dc.contributor.funder | Fundación ProCNIC | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2045-2322 | es_ES |
dc.relation.publisherversion | 10.1038/s41598-024-60158-8 | es_ES |
dc.identifier.journal | Scientific reports | es_ES |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Imagen Avanzada | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PID2021-123124OB-I00 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PID2020-115088RB-I00 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/DTS22/00030 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/S2017/BMD-3867/RENIM-CM | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/SEV-2015-0505 | es_ES |