Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/17952
Título
Xenopus HJURP and condensin II are required for CENP-A assembly.
Autor(es)
Fecha de publicación
2011-02-21
Cita
J Cell Biol . 2011 ;192(4):569-82.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Centromeric protein A (CENP-A) is the epigenetic mark of centromeres. CENP-A replenishment is necessary in each cell cycle to compensate for the dilution associated to DNA replication, but how this is achieved mechanistically is largely unknown. We have developed an assay using Xenopus egg extracts that can recapitulate the spatial and temporal specificity of CENP-A deposition observed in human cells, providing us with a robust in vitro system amenable to molecular dissection. Here we show that this deposition depends on Xenopus Holliday junction-recognizing protein (xHJURP), a member of the HJURP/Scm3 family recently identified in yeast and human cells, further supporting the essential role of these chaperones in CENP-A loading. Despite little sequence homology, human HJURP can substitute for xHJURP. We also report that condensin II, but not condensin I, is required for CENP-A assembly and contributes to retention of centromeric CENP-A nucleosomes both in mitosis and interphase. We propose that the chromatin structure imposed by condensin II at centromeres enables CENP-A incorporation initiated by xHJURP.
MESH
Adenosine Triphosphatases | Animals | Autoantigens | Centromere | Centromere Protein A | Chromatin | Chromosomal Proteins, Non-Histone | DNA-Binding Proteins | Epigenesis, Genetic | Histone Chaperones | Humans | Interphase | Multiprotein Complexes | Xenopus Proteins | Xenopus laevis
Versión en línea
DOI
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