dc.contributor.author | Viera, Alberto | |
dc.contributor.author | Berenguer, Inés | |
dc.contributor.author | Ruiz-Torres, Miguel | |
dc.contributor.author | Gómez, Rocío | |
dc.contributor.author | Guajardo, Andrea | |
dc.contributor.author | Barbero, José Luis | |
dc.contributor.author | Losada, Ana | |
dc.contributor.author | Suja, José A | |
dc.date.accessioned | 2024-02-08T17:27:57Z | |
dc.date.available | 2024-02-08T17:27:57Z | |
dc.date.issued | 2020-06-04 | |
dc.identifier.citation | EMBO Rep . 2020;21(6):e49273. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/17656 | |
dc.description.abstract | Cohesin cofactors regulate the loading, maintenance, and release of cohesin complexes from chromosomes during mitosis but little is known on their role during vertebrate meiosis. One such cofactor is PDS5, which exists as two paralogs in somatic and germline cells, PDS5A and PDS5B, with unclear functions. Here, we have analyzed their distribution and functions in mouse spermatocytes. We show that simultaneous excision of Pds5A and Pds5B results in severe defects during early prophase I while their individual depletion does not, suggesting their functional redundancy. Shortened axial/lateral elements and a reduction of early recombination nodules are observed after the strong depletion of PDS5A/B proteins. Moreover, telomere integrity and their association to the nuclear envelope are severely compromised. As these defects occur without detectable reduction in chromosome-bound cohesin, we propose that the dynamic behavior of the complex, mediated by PDS5 proteins, is key for successful completion of meiotic prophase I. | es_ES |
dc.description.sponsorship | We express our sincere thanks to John Schimenti and Rolf Jessberger for providing REC8 and SMC1 beta knock out male mice, respectively, and to Jibak Lee, Mary Ann Handel, Attila Toth, and Manfred Alsheimer for providing antibodies. We also thank Lorena Barreras for expert histological assistance and Miriam Rodriguez for mouse genotyping. This work was supported by the Spanish Ministry of Science and Innovation, State Research Agency (Agencia Estatal de Investigacion, AEI), and European Regional Development Funds (ERDF) through grants BFU2014-53681-P (to JAS), BFU2016-79841-R (to AL), BFU2017-89408-R, FPI "Severo Ochoa" fellowship to MR-T and Centro de Excelencia "Severo Ochoa" to CNIO, "Ayuda para el Fomento de la Investigacion en Estudios de Master", and "Ayudas PostMaster del Departamento de Biologia" from Universidad Autonoma de Madrid fellowships to AG. CNIO is supported by the Instituto de Salud Carlos III (ISCIII). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | EMBO Press | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject.mesh | Meiosis | es_ES |
dc.subject.mesh | Telomere | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Cell Cycle Proteins | es_ES |
dc.subject.mesh | Male | es_ES |
dc.subject.mesh | Mice | es_ES |
dc.subject.mesh | Mitosis | es_ES |
dc.subject.mesh | Spermatocytes | es_ES |
dc.subject.mesh | Synaptonemal Complex | es_ES |
dc.title | PDS5 proteins regulate the length of axial elements and telomere integrity during male mouse meiosis. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.identifier.pubmedID | 32285610 | es_ES |
dc.format.volume | 21 | es_ES |
dc.format.number | 6 | es_ES |
dc.format.page | e49273 | es_ES |
dc.identifier.doi | 10.15252/embr.201949273 | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1469-3178 | es_ES |
dc.relation.publisherversion | https://doi.org/10.15252/embr.201949273. | es_ES |
dc.identifier.journal | EMBO reports | es_ES |
dc.repisalud.institucion | CNIO | es_ES |
dc.repisalud.orgCNIO | CNIO::Grupos de investigación::Grupo de Dinámica Cromosómica | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/BFU2014-53681-P | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/BFU2017-89408-R | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/BFU2014-53681-P | es_ES |
dc.relation.projectFIS | info:eu-repo/grantAgreement/ES/BFU2016-79841-R | es_ES |