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dc.contributor.author | D'Amico, Gabriela | |
dc.contributor.author | Jones, Dylan T | |
dc.contributor.author | Nye, Emma | |
dc.contributor.author | Sapienza, Karen | |
dc.contributor.author | Ramjuan, Antoine R | |
dc.contributor.author | Reynolds, Louise E | |
dc.contributor.author | Robinson, Stephen D | |
dc.contributor.author | Kostourou, Vassiliki | |
dc.contributor.author | Martinez Garcia, Maria Dolores | |
dc.contributor.author | Aubyn, Deborah | |
dc.contributor.author | Grose, Richard | |
dc.contributor.author | Thomas, Gareth J | |
dc.contributor.author | Spencer-Dene, Bradley | |
dc.contributor.author | Zicha, Daniel | |
dc.contributor.author | Davies, Derek | |
dc.contributor.author | Tybulewicz, Victor | |
dc.contributor.author | Hodivala-Dilke, Kairbaan M | |
dc.date.accessioned | 2024-02-01T12:12:18Z | |
dc.date.available | 2024-02-01T12:12:18Z | |
dc.date.issued | 2009-12 | |
dc.identifier.citation | Development . 2009;136(23):4043-53. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/17410 | |
dc.description.abstract | Sprouting angiogenesis and lymphatic-blood vessel segregation both involve the migration of endothelial cells, but the precise migratory molecules that govern the decision of blood vascular endothelial cells to segregate into lymphatic vasculature are unknown. Here, we deleted endothelial Rac1 in mice (Tie1-Cre(+);Rac1(fl/fl)) and revealed, unexpectedly, that whereas blood vessel morphology appeared normal, lymphatic-blood vessel separation was impaired, with corresponding edema, haemorrhage and embryonic lethality. Importantly, normal levels of Rac1 were essential for directed endothelial cell migratory responses to lymphatic-inductive signals. Our studies identify Rac1 as a crucial part of the migratory machinery required for endothelial cells to separate and form lymphatic vasculature. | es_ES |
dc.description.sponsorship | We thank Dr Christiana Ruhrberg for assistance with the hindbrain model; Mitchel Germain for assistance with experiments; Garry Saunders, Colin Wren, Colin Pegrum, Claire Darnborough, Clare Watkins and Emma Murray for technical assistance with animals; Drs John Collard and Vania Braga for providing the GST-PAK-CRIB construct; Prof. Dietmar Vestweber for providing the endomucin antibody; Prof. Reinhard Fässler for providing Tie1-Cre mice; Prof. Kari Alitalo and Dr Tuomas Tammela for providing Vegfr3+/- mice; Takako Makita for technical advice on the India ink injections; and Prof. Peter Mortimer, Prof. Ian Hart and Prof. Tanya Petrova for their critical comments on the manuscript. G.D., E.N., A.R.R., S.D.R., L.E.R., V.K., K.S., D.M., D.A., B.S.-D., D.Z., D.D. and K.M.H.-D. were funded by Cancer Research UK. R.G. was funded by The Wellcome Trust. V.T. was funded by The Medical Research Council. D.T.J. was funded by Breast Cancer Campaign. G.T. was funded and by The Health Foundation, Royal College of Pathologists. Deposited in PMC for release after 6 months. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | The Company of Biologists | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject.mesh | Gene Expression Regulation, Developmental | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Blood Vessels | es_ES |
dc.subject.mesh | Cell Separation | es_ES |
dc.subject.mesh | Cells, Cultured | es_ES |
dc.subject.mesh | Embryo, Mammalian | es_ES |
dc.subject.mesh | Endothelial Cells | es_ES |
dc.subject.mesh | Endothelium, Vascular | es_ES |
dc.subject.mesh | Fluorescent Antibody Technique, Direct | es_ES |
dc.subject.mesh | Fluorescent Dyes | es_ES |
dc.subject.mesh | Galactosides | es_ES |
dc.subject.mesh | Gene Deletion | es_ES |
dc.subject.mesh | Immunohistochemistry | es_ES |
dc.subject.mesh | Indoles | es_ES |
dc.subject.mesh | Lymphatic Vessels | es_ES |
dc.subject.mesh | Mice | es_ES |
dc.subject.mesh | Mice, Transgenic | es_ES |
dc.subject.mesh | Neovascularization, Physiologic | es_ES |
dc.subject.mesh | RNA, Small Interfering | es_ES |
dc.subject.mesh | Receptor, TIE-2 | es_ES |
dc.subject.mesh | Transfection | es_ES |
dc.subject.mesh | beta-Galactosidase | es_ES |
dc.subject.mesh | rac1 GTP-Binding Protein | es_ES |
dc.title | Regulation of lymphatic-blood vessel separation by endothelial Rac1. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.identifier.pubmedID | 19906871 | es_ES |
dc.format.volume | 136 | es_ES |
dc.format.number | 23 | es_ES |
dc.format.page | 4043 | es_ES |
dc.identifier.doi | 10.1242/dev.035014 | es_ES |
dc.contributor.funder | Wellcome Trust | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1477-9129 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1242/dev.035014. | es_ES |
dc.identifier.journal | Development (Cambridge, England) | es_ES |
dc.repisalud.institucion | CNIO | es_ES |
dc.repisalud.orgCNIO | CNIO::Unidades técnicas::Unidad de Citometría de Flujo | es_ES |
dc.rights.accessRights | open access | es_ES |
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