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dc.contributor.authorAizarna-Lopetegui, Uxue
dc.contributor.authorGarcía-Astrain, Clara
dc.contributor.authorRenero-Lecuna, Carlos
dc.contributor.authorGonzález-Callejo, Patricia
dc.contributor.authorVillaluenga, Irune
dc.contributor.authordel Pozo, Miguel Angel 
dc.contributor.authorSánchez-Álvarez, Miguel
dc.contributor.authorHenriksen-Lacey, Malou
dc.contributor.authorJimenez de Aberasturi, Dorleta
dc.identifier.citationJ Mater Chem B. 2023 Oct 11;11(39):9431-9442.es_ES
dc.description.abstract3D-printed cell models are currently in the spotlight of medical research. Whilst significant advances have been made, there are still aspects that require attention to achieve more realistic models which faithfully represent the in vivo environment. In this work we describe the production of an artery model with cyclic expansive properties, capable of mimicking the different physical forces and stress factors that cells experience in physiological conditions. The artery wall components are reproduced using 3D printing of thermoresponsive polymers with inorganic nanoparticles (NPs) representing the outer tunica adventitia, smooth muscle cells embedded in extracellular matrix representing the tunica media, and finally a monolayer of endothelial cells as the tunica intima. Cyclic expansion can be induced thanks to the inclusion of photo-responsive plasmonic NPs embedded within the thermoresponsive ink composition, resulting in changes in the thermoresponsive polymer hydration state and hence volume, in a stimulated on-off manner. By changing the thermoresponsive polymer composition, the transition temperature and pulsatility can be efficiently tuned. We show the direct effect of cyclic expansion and contraction on the overlying cell layers by analyzing transcriptional changes in mechanoresponsive mesenchymal genes associated with such microenvironmental physical cues. The technique described herein involving stimuli-responsive 3D printed tissue constructs, also described as four- dimensional (4D) printing, offers a novel approach for the production of dynamic biomodels.es_ES
dc.description.sponsorshipFinancial support is acknowledged from the MCIN/AEI/ 10.13039/501100011033 through grant # PID2019-108854RAI00. C. G. A. thanks to the Ministerio de Ciencia e Innovacio´n (MCIN) for a Juan de la Cierva Incorporacio´n Fellowship (IJC2019-040827-I). M. S.-A. is recipient of a Ramo´n y Cajal contract and a ‘‘Generacio´n de Conocimiento’’ grant from the Ministerio de Ciencia e Innovacio´n (RYC2020-029690-I and PID2021-128106NA-I00). MAdP is coordinator and PL of ‘‘AtheroConvergence’’ La Caixa Foundation Health Research consortium (HR20-00075). The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the MCIN and the Pro CNIC Foundation, and is a Severo Ochoa Center of Excellence (grant CEX2020-001041-S). We acknowledge ALBA for provision of synchrotron radiation facilities. We would like to thank Dr Marc Malfois for assistance in using BL11-NCD beamline, and Unai Cossı´o and Daniel Padro for help with image analysis.es_ES
dc.publisherRoyal Society of Chemistry (RSC) es_ES
dc.subject.meshEndothelial Cells es_ES
dc.subject.meshNanoparticles es_ES
dc.subject.meshPolymers es_ES
dc.subject.meshExtracellular Matrix es_ES
dc.subject.meshArteries es_ES
dc.titleRemodeling arteries: studying the mechanical properties of 3D-bioprinted hybrid photoresponsive materials.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.journalJournal of materials chemistry. Bes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Mecanoadaptación y Biología de Caveolases_ES
dc.rights.accessRightsopen accesses_ES

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
This item is licensed under a: Attribution-NonCommercial-NoDerivatives 4.0 Internacional