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dc.contributor.author | Polo-Generelo, Salvador | |
dc.contributor.author | Torres, Belén | |
dc.contributor.author | Guerrero-Martínez, José A | |
dc.contributor.author | Camafeita, Emilio | |
dc.contributor.author | Vazquez, Jesus | |
dc.contributor.author | Reyes, José C | |
dc.contributor.author | Pintor-Toro, José A | |
dc.date.accessioned | 2023-11-06T12:25:42Z | |
dc.date.available | 2023-11-06T12:25:42Z | |
dc.date.issued | 2022-09-15 | |
dc.identifier.citation | Noncoding RNA. 2022 Sep 15;8(5):62. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/16644 | |
dc.description.abstract | Long non-coding RNAs (lncRNAs) have emerged as key regulators in a wide range of biological processes. Here, we identified a mouse miRNA-host gene lncRNA (lnc-Nr6a1) upregulated early during epithelial-to-mesenchymal transition (EMT). We show that when lncRNA is processed, it gives rise to two abundant polyadenylated isoforms, lnc-Nr6a1-1 and lnc-Nr6a1-2, and a longer non-polyadenylated microprocessor-driven lnc-pri-miRNA containing clustered pre-miR-181a2 and pre-miR-181b2 hairpins. Ectopic expression of the lnc-Nr6a1-1 or lnc-Nr6a1-2 isoform enhanced cell migration and the invasive capacity of the cells, whereas the expression of the isoforms and miR-181a2 and miR-181b2 conferred anoikis resistance. Lnc-Nr6a1 gene deletion resulted in cells with lower adhesion capacity and reduced glycolytic metabolism, which are restored by lnc-Nr6a1-1 isoform expression. We performed identification of direct RNA interacting proteins (iDRIP) to identify proteins interacting directly with the lnc-Nr6a1-1 isoform. We defined a network of interacting proteins, including glycolytic enzymes, desmosome proteins and chaperone proteins; and we demonstrated that the lnc-Nr6a1-1 isoform directly binds and acts as a scaffold molecule for the assembly of ENO1, ALDOA, GAPDH, and PKM glycolytic enzymes, along with LDHA, supporting substrate channeling for efficient glycolysis. Our results unveil a role of Lnc-Nr6a1 as a multifunctional lncRNA acting as a backbone for multiprotein complex formation and primary microRNAs. | es_ES |
dc.description.sponsorship | This research was funded by Ministerio de Economía y Competitividad, grant number SAF2017-861892-P and PID2020-119732RB-100 to J.A.P.-T. and PGC2018-097019-B-100 and PID2021- 122348NB-100 to J.V., and by la Caixa Banking Foundation, project codes HR17-00247 and HR22-00253 to J.V. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | TGF-β-Upregulated Lnc-Nr6a1 Acts as a Reservoir of miR-181 and Mediates Assembly of a Glycolytic Complex. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 36136852 | es_ES |
dc.format.volume | 8 | es_ES |
dc.format.number | 5 | es_ES |
dc.identifier.doi | 10.3390/ncrna8050062 | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | es_ES |
dc.contributor.funder | Fundación La Caixa | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2311-553X | es_ES |
dc.relation.publisherversion | 10.3390/ncrna8050062 | es_ES |
dc.identifier.journal | Non-coding RNA | es_ES |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Proteómica | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/SAF2017-861892-P | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PID2020-119732RB-100 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PGC2018-097019-B-100 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PID2021-122348NB-100 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/HR17-00247 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/HR22-00253 | es_ES |