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dc.contributor.author | Campos-Iglesias, Diana | |
dc.contributor.author | Fraile, Julia M | |
dc.contributor.author | Bretones, Gabriel | |
dc.contributor.author | Montero, Alejandro A | |
dc.contributor.author | Bonzon-Kulichenko, Elena | |
dc.contributor.author | Vazquez, Jesus | |
dc.contributor.author | López-Otín, Carlos | |
dc.contributor.author | Freije, José M P | |
dc.date.accessioned | 2023-09-15T09:22:06Z | |
dc.date.available | 2023-09-15T09:22:06Z | |
dc.date.issued | 2023-01-26 | |
dc.identifier.citation | Cell Death Dis. 2023 Jan 26;14(1):60. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/16466 | |
dc.description.abstract | The spindle assembly checkpoint (SAC) is an essential mechanism that ensures the accurate chromosome segregation during mitosis, thus preventing genomic instability. Deubiquitinases have emerged as key regulators of the SAC, mainly by determining the fate of proteins during cell cycle progression. Here, we identify USP49 deubiquitinase as a novel regulator of the spindle checkpoint. We show that loss of USP49 in different cancer cell lines impairs proliferation and increases aneuploidy. In addition, USP49-depleted cells overcome the arrest induced by the SAC in the presence of nocodazole. Finally, we report new binding partners of USP49, including ribophorin 1, USP44, and different centrins. | es_ES |
dc.description.sponsorship | We thank A.P. Ugalde, D. Rodríguez, J.G. Pérez-Silva, Y. Español and F. Rodríguez for helpful comments and advice. We also thank S.A. Miranda, D. Álvarez-Puente and C. Garabaya for excellent technical assistance, as well as the staff of the scientific core facilities from the University of Oviedo (Unidad de Ensayos Biotecnológicos y Biomédicos, Servicios Científico-Técnicos). This work was supported by the Ministerio de Ciencia e Innovación (SAF2017-87655-R, PDI2020-118394RB-100 and PGC2018- 097019-B-I00), “la Caixa” Banking Foundation (project code HR17-00247), and the European Research Council (742067, DeAge). The IUOPA is funded by the Asturian Government and Fundación Cajastur-Liberbank. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | M Phase Cell Cycle Checkpoints | es_ES |
dc.subject.mesh | Spindle Apparatus | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Aneuploidy | es_ES |
dc.subject.mesh | Mitosis | es_ES |
dc.subject.mesh | Deubiquitinating Enzymes | es_ES |
dc.subject.mesh | Cell Cycle Proteins | es_ES |
dc.subject.mesh | Ubiquitin Thiolesterase | es_ES |
dc.title | USP49 deubiquitinase regulates the mitotic spindle checkpoint and prevents aneuploidy. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 36702832 | es_ES |
dc.format.volume | 14 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 60 | es_ES |
dc.identifier.doi | 10.1038/s41419-023-05600-x | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Fundación La Caixa | es_ES |
dc.contributor.funder | Unión Europea. Comisión Europea. European Research Council (ERC) | es_ES |
dc.contributor.funder | Fundación Cajastur-Liberbank | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2041-4889 | es_ES |
dc.relation.publisherversion | 10.1038/s41419-023-05600-x | es_ES |
dc.identifier.journal | Cell death & disease | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Proteómica cardiovascular | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/ERC/742067 | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/SAF2017-87655-R | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PDI2020-118394RB-100 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PGC2018-097019-B-I00 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/HR17-00247 | es_ES |