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dc.contributor.authorDel Monte-Monge, Alberto
dc.contributor.authorMartos-Folgado, Inmaculada
dc.contributor.authorRodríguez-Ronchel, Ana
dc.contributor.authorRamiro, Almudena R 
dc.date.accessioned2023-07-17T13:00:32Z
dc.date.available2023-07-17T13:00:32Z
dc.date.issued2023-04-30
dc.identifier.citationSTAR Protoc. 2023 Apr 30;4(2):102274es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/16278
dc.description.abstractThe antibody immune response plays a critical role in atherosclerosis. Here, we present a protocol for assessing the impact of an antigen-specific germinal center antibody response on atherosclerosis development, using a pro-atherogenic mouse model deficient for the production of germinal-center-derived antibodies. We describe steps for bone marrow transfer from donor mice into irradiated recipient mice. We then detail immunization of mouse chimeras with atheroprotective malondialdehyde low-density lipoprotein during high-fat diet feeding and atherosclerosis burden analysis. For complete details on the use and execution of this protocol, please refer to Martos-Folgado et al. (2022).1.es_ES
dc.description.sponsorshipWe thank all members of the B Cell Biology Laboratory for useful discussions. A.D.M.M. is funded by ‘‘la Caixa’’ Foundation HR17-00247, I.M.-F. was a fellow of the research training program funded by Ministerio de Economía y Competitividad (SVP-2014-068216), A.R-R. is a fellow of the research training program funded by Ministerio de Ciencia, Innovacio´ n y Universidades (PRE2020-091873), and A.R. is supported by Centro Nacional de Investigaciones Cardiovasculares (CNIC). The project leading to these results has received funding from ‘‘la Caixa’’ Foundation under the project code HR17-00247 and HR22-0253 and from SAF2016-75511-R and PID2019-106773RB-I00/AEI/ 10.13039/501100011033 grants to A.R.R. (Plan Estatal de Investigación Científica y Técnica y de Innovación 2013–2016 Programa Estatal de I+D+i Orientada a los Retos de la Sociedad Retos Investigación: Proyectos I + D + i 2016, Ministerio de Economía, Industria y Competitividad) and co-funding by Fondo Europeo de Desarrollo Regional (FEDER). The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación (MCIN), and the Pro CNIC Foundation and is a Severo Ochoa institute (CEX2020-001041-S grant funded by MCIN/AEI/ 10.13039/501100011033).es_ES
dc.language.isoenges_ES
dc.publisherCell Press es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleAssessing the impact of an antigen-specific antibody response on atherosclerosis development in mice.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID37126444es_ES
dc.format.volume4es_ES
dc.format.number2es_ES
dc.format.page102274es_ES
dc.identifier.doi10.1016/j.xpro.2023.102274es_ES
dc.contributor.funderFundación La Caixa es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España) es_ES
dc.contributor.funderMinisterio de Ciencia, Innovación y Universidades (España) es_ES
dc.contributor.funderCentro Nacional de Investigaciones Cardiovasculares Carlos III (España) es_ES
dc.contributor.funderMinisterio de Economía, Industria y Competitividad (España) es_ES
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España) es_ES
dc.contributor.funderFundación ProCNIC es_ES
dc.contributor.funderMinisterio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España) es_ES
dc.description.peerreviewedSíes_ES
dc.identifier.e-issn2666-1667es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.xpro.2023.102274es_ES
dc.identifier.journalSTAR protocolses_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Biología de linfocitos Bes_ES
dc.repisalud.institucionCNICes_ES
dc.rights.accessRightsopen accesses_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/HR17-00247es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/SVP-2014-068216es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PRE2020-091873es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/SAF2016-75511-Res_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/PID2019-106773RB-I00/AEI/10.13039/501100011033es_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/CEX2020-001041-Ses_ES
dc.relation.projectFECYTinfo:eu-repo/grantAgreement/ES/MCIN/AEI/10.13039/501100011033es_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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