dc.contributor.author | Del Monte-Monge, Alberto | |
dc.contributor.author | Martos-Folgado, Inmaculada | |
dc.contributor.author | Rodríguez-Ronchel, Ana | |
dc.contributor.author | Ramiro, Almudena R | |
dc.date.accessioned | 2023-07-17T13:00:32Z | |
dc.date.available | 2023-07-17T13:00:32Z | |
dc.date.issued | 2023-04-30 | |
dc.identifier.citation | STAR Protoc. 2023 Apr 30;4(2):102274 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/16278 | |
dc.description.abstract | The antibody immune response plays a critical role in atherosclerosis. Here, we present a protocol for assessing the impact of an antigen-specific germinal center antibody response on atherosclerosis development, using a pro-atherogenic mouse model deficient for the production of germinal-center-derived antibodies. We describe steps for bone marrow transfer from donor mice into irradiated recipient mice. We then detail immunization of mouse chimeras with atheroprotective malondialdehyde low-density lipoprotein during high-fat diet feeding and atherosclerosis burden analysis. For complete details on the use and execution of this protocol, please refer to Martos-Folgado et al. (2022).1. | es_ES |
dc.description.sponsorship | We thank all members of the B Cell Biology Laboratory for useful discussions. A.D.M.M. is funded by
‘‘la Caixa’’ Foundation HR17-00247, I.M.-F. was a fellow of the research training program funded by
Ministerio de Economía y Competitividad (SVP-2014-068216), A.R-R. is a fellow of the research
training program funded by Ministerio de Ciencia, Innovacio´ n y Universidades (PRE2020-091873),
and A.R. is supported by Centro Nacional de Investigaciones Cardiovasculares (CNIC). The project
leading to these results has received funding from ‘‘la Caixa’’ Foundation under the project code
HR17-00247 and HR22-0253 and from SAF2016-75511-R and PID2019-106773RB-I00/AEI/
10.13039/501100011033 grants to A.R.R. (Plan Estatal de Investigación Científica y Técnica y de
Innovación 2013–2016 Programa Estatal de I+D+i Orientada a los Retos de la Sociedad Retos
Investigación: Proyectos I + D + i 2016, Ministerio de Economía, Industria y Competitividad) and
co-funding by Fondo Europeo de Desarrollo Regional (FEDER). The CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación (MCIN), and the Pro CNIC
Foundation and is a Severo Ochoa institute (CEX2020-001041-S grant funded by MCIN/AEI/
10.13039/501100011033). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Cell Press | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Assessing the impact of an antigen-specific antibody response on atherosclerosis development in mice. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.identifier.pubmedID | 37126444 | es_ES |
dc.format.volume | 4 | es_ES |
dc.format.number | 2 | es_ES |
dc.format.page | 102274 | es_ES |
dc.identifier.doi | 10.1016/j.xpro.2023.102274 | es_ES |
dc.contributor.funder | Fundación La Caixa | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | es_ES |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | es_ES |
dc.contributor.funder | Centro Nacional de Investigaciones Cardiovasculares Carlos III (España) | es_ES |
dc.contributor.funder | Ministerio de Economía, Industria y Competitividad (España) | es_ES |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Fundación ProCNIC | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2666-1667 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.xpro.2023.102274 | es_ES |
dc.identifier.journal | STAR protocols | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Biología de linfocitos B | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/HR17-00247 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/SVP-2014-068216 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PRE2020-091873 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/SAF2016-75511-R | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PID2019-106773RB-I00/AEI/10.13039/501100011033 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/CEX2020-001041-S | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/MCIN/AEI/10.13039/501100011033 | es_ES |