dc.contributor.author | Laveriano-Santos, Emily P | |
dc.contributor.author | Marhuenda-Muñoz, María | |
dc.contributor.author | Vallverdú-Queralt, Anna | |
dc.contributor.author | Martínez-Huélamo, Miriam | |
dc.contributor.author | Tresserra-Rimbau, Anna | |
dc.contributor.author | Miliarakis, Elefterios | |
dc.contributor.author | Arancibia-Riveros, Camila | |
dc.contributor.author | Jáuregui, Olga | |
dc.contributor.author | Ruiz-León, Ana María | |
dc.contributor.author | Castro-Baquero, Sara | |
dc.contributor.author | Estruch, Ramón | |
dc.contributor.author | Bodega, Patricia | |
dc.contributor.author | Miguel, Mercedes de | |
dc.contributor.author | Cos-Gandoy, Amaya de | |
dc.contributor.author | Martínez-Gómez, Jesús | |
dc.contributor.author | Santos-Beneit, Gloria | |
dc.contributor.author | Fernández-Alvira, Juan M | |
dc.contributor.author | Fernández-Jiménez, Rodrigo | |
dc.contributor.author | Lamuela-Raventós, Rosa M | |
dc.date.accessioned | 2023-04-03T14:14:57Z | |
dc.date.available | 2023-04-03T14:14:57Z | |
dc.date.issued | 2022-06-14 | |
dc.identifier.citation | Antioxidants (Basel). 2022 Jun 14;11(6):1167 | es_ES |
dc.identifier.issn | 2076-3921 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/15743 | |
dc.description.abstract | This study aimed to develop and validate a liquid chromatography/electrospray ionization-linear ion trap quadrupole-Orbitrap-high-resolution mass spectrometry (HPLC/ESI-LTQ-Orbitrap-HRMS) method to identify and quantify urinary microbial phenolic metabolites (MPM), as well as to explore the relationship between MPM and dietary (poly)phenols in Spanish adolescents. A total of 601 spot urine samples of adolescents aged 12.02 ± 0.41 years were analyzed. The quantitative method was validated for linearity, limit of detection, limit of quantification, recovery, intra- and inter-day accuracy and precision, as well as postpreparative stability according to the criteria established by the Association of Official Agricultural Chemists International. A total of 17 aglycones and 37 phase II MPM were identified and quantified in 601 spot urine samples. Phenolic acids were the most abundant urinary MPM, whereas stilbenes, hydroxytyrosol, and enterodiol were the least abundant. Urinary hydroxycoumarin acids (urolithins) were positively correlated with flavonoid and total (poly)phenol intake. An HPLC-ESI-LTQ-Orbitrap-HRMS method was developed and fully validated to quantify MPM. The new method was performed accurately and is suitable for MPM quantification in large epidemiological studies. Urinary lignans and urolithins are proposed as potential biomarkers of grain and nut intake in an adolescent population. | es_ES |
dc.description.sponsorship | This research was funded in part by CICYT (AGL2016-75329-R and PID2020-114022RBI00]), the Instituto de Salud Carlos III—ISCIII (CIBEROBN CB12/03/30020) from the Ministry of
Science, Innovation, and Universities (AEI/FEDER, UE) and Generalitat de Catalunya (GC) 2017
SGR 196. The SI! Program for Secondary School trial was funded by the Fundació la Marató de TV3
(369/C/2016), the SHE Foundation-‘la Caixa’ Foundation (LCF/PR/CE16/10700001), the Ministerio
de Ciencia e Innovación (AGL2016-75329-R). E.P.L.-S. is supported by the FI-SDUR (EMC/3345/2020)
fellowship from the Generalitat de Catalunya. M.M.-M. and S.C.-B. are supported by the Ministry
of Science, Innovation, and Universities for the FPU17/00513 and FPU17/00785 fellowship. A.V.-Q.
is supported the Ministry of Science, Innovation, and Universities for the Ramon y Cajal contract
(RYC-2016-19355). A.T.-R. is supported by Serra Húnter Fellowship JM-G is a postgraduate fellow
of the Ministerio de Ciencia e Innovación of Spain at the Residencia de Estudiantes (2020–ongoing).
G.S.-B. is supported by a “la Caixa” Foundation fellowship (LCF/PR/MS19/12220001). R.F.-J. is a
recipient of grant PI19/01704 funded by the Fondo de Investigación Sanitaria- Instituto de Salud
Carlos III (ISCIII) and co-funded by the European Regional Development Fund/European Social
Fund “A way to make Europe”/“Investing in your future”. The Centro Nacional de Investigaciones
Cardiovasculares is supported by the ISCIII, the Ministerio de Ciencia e Innovación, and the Pro
CNIC Foundation, and is a Severo Ochoa Center of Excellence (CEX2020-001041-S). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 35740067 | es_ES |
dc.format.volume | 11 | es_ES |
dc.format.number | 6 | es_ES |
dc.identifier.doi | 10.3390/antiox11061167 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | es_ES |
dc.contributor.funder | Fundación La Marató TV3 | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Fundación La Caixa | es_ES |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | es_ES |
dc.contributor.funder | Fundación ProCNIC | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.relation.publisherversion | 10.3390/antiox11061167 | es_ES |
dc.identifier.journal | Antioxidants (Basel, Switzerland) | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Imagen y Salud Cardiovascular | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PID2020-114022RBI00 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/CB12/03/30020 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/369/C/2016 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/LCF/PR/CE16/10700001 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/AGL2016-75329-R | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/EMC/3345/2020 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/LCF/PR/MS19/12220001 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/CEX2020-001041-S | es_ES |