dc.contributor.author | Díaz-Torres, Alfonsa | |
dc.contributor.author | Rosales-Nieves, Alicia E | |
dc.contributor.author | Pearson, John R | |
dc.contributor.author | Santa-Cruz Mateos, Carmen | |
dc.contributor.author | Marín-Menguiano, Miriam | |
dc.contributor.author | Marshall, Owen J | |
dc.contributor.author | Brand, Andrea H | |
dc.contributor.author | González-Reyes, Acaimo | |
dc.date.accessioned | 2022-12-09T16:58:31Z | |
dc.date.available | 2022-12-09T16:58:31Z | |
dc.date.issued | 2021-04-26 | |
dc.identifier.citation | Curr Biol . 2021 Apr 26;31(8):1744-1753.e5 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/15268 | |
dc.description.abstract | Stem cells reside in specialized microenvironments or niches that balance stem cell proliferation and differentiation.1,2 The extracellular matrix (ECM) is an essential component of most niches, because it controls niche homeostasis, provides physical support, and conveys extracellular signals.3-11 Basement membranes (BMs) are thin ECM sheets that are constituted mainly by Laminins, Perlecan, Collagen IV, and Entactin/Nidogen and surround epithelia and other tissues.12 Perlecans are secreted proteoglycans that interact with ECM proteins, ligands, receptors, and growth factors such as FGF, PDGF, VEGF, Hedgehog, and Wingless.13-18 Thus, Perlecans have structural and signaling functions through the binding, storage, or sequestering of specific ligands. We have used the Drosophila ovary to assess the importance of Perlecan in the functioning of a stem cell niche. Ovarioles in the adult ovary are enveloped by an ECM sheath and possess a tapered structure at their anterior apex termed the germarium. The anterior tip of the germarium hosts the germline niche, where two to four germline stem cells (GSCs) reside together with a few somatic cells: terminal filament cells (TFCs), cap cells (CpCs), and escort cells (ECs).19 We report that niche architecture in the developing gonad requires trol, that niche cells secrete an isoform-specific Perlecan-rich interstitial matrix, and that DE-cadherin-dependent stem cell-niche adhesion necessitates trol. Hence, we provide evidence to support a structural role for Perlecan in germline niche establishment during larval stages and in the maintenance of a normal pool of stem cells in the adult niche. | es_ES |
dc.description.sponsorship | This work was funded by the Spanish State Research Agency (MCUI/AEI,
http://www.ciencia.gob.es/; grant numbers BFU2015-65372-P, PGC2018-
097115-B-I00, and MDM-2016-0687 to A.G.-R.) and by the European Regional
Development Fund (http://ec.europa.eu/regional_policy/en/funding/erdf/).
A.H.B. was supported by the Royal Society Darwin Trust Research Professorship and Wellcome Trust Senior Investigator Award 103792. A.H.B. acknowledges core funding to the Gurdon Institute from the Wellcome Trust (092096)
and CRUK (C6946/A14492). O.J.M. was supported by an EMBO long-term
fellowship (ALTF 423-2011). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Cell Press | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | Drosophila | es_ES |
dc.subject.mesh | Stem Cell Niche | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Drosophila Proteins | es_ES |
dc.subject.mesh | Drosophila melanogaster | es_ES |
dc.subject.mesh | Female | es_ES |
dc.subject.mesh | Heparan Sulfate Proteoglycans | es_ES |
dc.subject.mesh | Ligands | es_ES |
dc.subject.mesh | Ovary | es_ES |
dc.title | Stem cell niche organization in the Drosophila ovary requires the ECM component Perlecan. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 33621481 | es_ES |
dc.format.volume | 31 | es_ES |
dc.format.number | 8 | es_ES |
dc.format.page | 1744 | es_ES |
dc.identifier.doi | 10.1016/j.cub.2021.01.071 | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación (España) | es_ES |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | es_ES |
dc.contributor.funder | Royal Society Darwin Trust Research Professorship | es_ES |
dc.contributor.funder | Wellcome Trust | es_ES |
dc.contributor.funder | European Molecular Biology Organization | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1879-0445 | es_ES |
dc.relation.publisherversion | 10.1016/j.cub.2021.01.071 | es_ES |
dc.identifier.journal | Current biology : CB | es_ES |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Microscopía | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/ALTF 423-2011 | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/BFU2015-65372-P | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PGC2018-097115-B-I00 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/MDM-2016-0687 | es_ES |