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dc.contributor.author | Portela, Marta | |
dc.contributor.author | Jimenez-Carretero, Daniel | |
dc.contributor.author | Labrador, Veronica | |
dc.contributor.author | Andreu, Maria Jose | |
dc.contributor.author | Arza, Elvira | |
dc.contributor.author | Caiolfa, Valeria R | |
dc.contributor.author | Manzanares, Miguel | |
dc.date.accessioned | 2022-12-09T16:41:42Z | |
dc.date.available | 2022-12-09T16:41:42Z | |
dc.date.issued | 2022-08-15 | |
dc.identifier.citation | Biol Open. 2022 Aug 15;11(8):bio059401 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/15267 | |
dc.description.abstract | Most studies addressing chromatin behaviour during preimplantation development are based on biochemical assays that lack spatial and cell-specific information, crucial during early development. Here, we describe the changes in chromatin taking place at the transition from totipotency to lineage specification, by using direct stochastical optical reconstruction microscopy (dSTORM) in whole-mount embryos during the first stages of mouse development. Through the study of two post-translational modifications of Histone 3 related to active and repressed chromatin, H3K4me3 and H3K9me3 respectively, we obtained a time-course of chromatin states, showing spatial differences between cell types, related to their differentiation state. This analysis adds a new layer of information to previous biochemical studies and provides novel insight to current models of chromatin organisation during the first stages of development. | es_ES |
dc.description.sponsorship | This work was supported by grants BFU2017-84914-P and PID2020-115755GB-I00 from the Spanish State Research Agency (AEI). M.P. was supported by fellowships from the Comunidad de Madrid (PEJD-2018-PRE/BMD-9079) and the Ministerio de Ciencia e Innovación (PRE2018-083477). The CBMSO is supported by an Institutional grant from the Fundación Ramon Areces, and the the CNIC is supported by the Instituto de Salud Carlos III (ISCIII), the Ministerio de Ciencia e Innovación (MCIN) and the Pro CNIC Foundation), and is a Severo Ochoa Center of Excellence grant (CEX2020-001041-S funded by MICIN/AEI/10.13039/501100011033). Deposited in PMC for immediate release. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | The Company of Biologists | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | Chromatin | es_ES |
dc.subject.mesh | Microscopy | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Embryo, Mammalian | es_ES |
dc.subject.mesh | Embryonic Development | es_ES |
dc.subject.mesh | Mice | es_ES |
dc.title | Chromatin dynamics through mouse preimplantation development revealed by single molecule localisation microscopy. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 35876820 | es_ES |
dc.format.volume | 11 | es_ES |
dc.format.number | 8 | es_ES |
dc.identifier.doi | 10.1242/bio.059401 | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación (España) | es_ES |
dc.contributor.funder | Comunidad de Madrid (España) | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Fundación Ramón Areces | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Fundación ProCNIC | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación. Centro de Excelencia Severo Ochoa (España) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2046-6390 | es_ES |
dc.identifier.journal | Biology open | es_ES |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Microscopía | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/BFU2017-84914 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PID2020-115755GB-I00 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PEJD-2018-PRE/BMD-9079 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PRE2018-083477 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/CEX2020-001041-S | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/MICIN/AEI/10.13039/501100011033 | es_ES |