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dc.contributor.author | Delgado, Irene | |
dc.contributor.author | Giovinazzo, Giovanna | |
dc.contributor.author | Temiño, Susana | |
dc.contributor.author | Gauthier, Yves | |
dc.contributor.author | Balsalobre, Aurelio | |
dc.contributor.author | Drouin, Jacques | |
dc.contributor.author | Torres, Miguel | |
dc.date.accessioned | 2022-12-01T15:02:17Z | |
dc.date.available | 2022-12-01T15:02:17Z | |
dc.date.issued | 2021-05-25 | |
dc.identifier.citation | Nat Commun. 2021 May 25;12(1):3086. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/15251 | |
dc.description.abstract | Meis1 and Meis2 are homeodomain transcription factors that regulate organogenesis through cooperation with Hox proteins. Elimination of Meis genes after limb induction has shown their role in limb proximo-distal patterning; however, limb development in the complete absence of Meis function has not been studied. Here, we report that Meis1/2 inactivation in the lateral plate mesoderm of mouse embryos leads to limb agenesis. Meis and Tbx factors converge in this function, extensively co-binding with Tbx to genomic sites and co-regulating enhancers of Fgf10, a critical factor in limb initiation. Limbs with three deleted Meis alleles show proximal-specific skeletal hypoplasia and agenesis of posterior skeletal elements. This failure in posterior specification results from an early role of Meis factors in establishing the limb antero-posterior prepattern required for Shh activation. Our results demonstrate roles for Meis transcription factors in early limb development and identify their involvement in previously undescribed interaction networks that regulate organogenesis. | es_ES |
dc.description.sponsorship | We thank members of the Torres group for stimulating discussions and suggestions. RNA probes were kindly provided by Marian Ros, Paola Bovolenta, and Jose Luis de la Pompa. We thank Keith Humphries and Hesham Sadek for providing the Meis1 floxed mice and Susan Mackem for the Hoxa6CreERT2 mice. We thank Alberto Benguría from the Genomics Unit, Manuel José Gómez from the Bioinformatics Unit, and Angeles Sanguino from the Pluripotent Cells Technology Unit. This study was supported by grant PGC2018-096486-B-I00 from the Spanish Ministerio de Ciencia e Innovación. The CNIC is supported by the Ministerio de Ciencia e Innovación and the Pro CNIC Foundation. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Basic Helix-Loop-Helix Transcription Factors | es_ES |
dc.subject.mesh | Body Patterning | es_ES |
dc.subject.mesh | DNA-Binding Proteins | es_ES |
dc.subject.mesh | Fibroblast Growth Factor 10 | es_ES |
dc.subject.mesh | Hedgehog Proteins | es_ES |
dc.subject.mesh | Homeodomain Proteins | es_ES |
dc.subject.mesh | Limb Buds | es_ES |
dc.subject.mesh | Mice, Knockout | es_ES |
dc.subject.mesh | Mice, Transgenic | es_ES |
dc.subject.mesh | Models, Genetic | es_ES |
dc.subject.mesh | Myeloid Ecotropic Viral Integration Site 1 Protein | es_ES |
dc.subject.mesh | Neoplasm Proteins | es_ES |
dc.subject.mesh | T-Box Domain Proteins | es_ES |
dc.subject.mesh | Transcription Factors | es_ES |
dc.subject.mesh | Transcriptional Activation | es_ES |
dc.title | Control of mouse limb initiation and antero-posterior patterning by Meis transcription factors. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 34035267 | es_ES |
dc.format.volume | 12 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 3086 | es_ES |
dc.identifier.doi | 10.1038/s41467-021-23373-9 | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Fundación ProCNIC | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2041-1723 | es_ES |
dc.relation.publisherversion | 10.1038/s41467-021-23373-9 | es_ES |
dc.identifier.journal | Nature communications | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Control Genético del Desarrollo y Regeneración de Órganos | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PGC2018-096486-B-I00 | es_ES |