Show simple item record

dc.contributor.authorZaragoza, Carlos 
dc.contributor.authorLopez-Rivera, Esther
dc.contributor.authorGarcia-Rama, Concepcion
dc.contributor.authorSaura, Marta
dc.contributor.authorMartinez-Ruiz, Antonio
dc.contributor.authorLizarbe, Tania R
dc.contributor.authorMartín-de-Lara, Fernando
dc.contributor.authorLamas, Santiago
dc.date.accessioned2021-02-17T09:02:52Z
dc.date.available2021-02-17T09:02:52Z
dc.date.issued2006-05-01
dc.identifier.citationJ Cell Sci. 2006; 119(Pt 9):1896-902es_ES
dc.identifier.issn0021-9533
dc.identifier.urihttp://hdl.handle.net/20.500.12105/11919
dc.description.abstractDuring bone development, osteoblast differentiation requires remodeling of the extracellular matrix. Although underlying mechanisms have not been elucidated, evidence points to the participation of the nitric oxide (NO) and cyclic guanosine 3',5'-monophosphate (cGMP) system. Here, we detected increased matrix metalloproteinase (MMP)-13 mRNA, protein and activity, as well as increased inducible NO synthase (iNOS) and NO production during the differentiation of MC3T3-E1 osteoblasts. Transcriptional activity of the MMP-13 promoter was augmented by NO, 8-bromo-cGMP (8-Br-cGMP), and by a dominant-positive form of protein kinase G (PKG1-alpha). The stimulatory effect on the MMP-13 promoter was partially inhibited by mutation of the osteoblast-specific element 2 (OSE-2) binding site. Core binding factor-1 (Cbfa-1) expression peaked at 7 days of differentiation, and was phosphorylated by PKG in vitro. Cbfa-1 was localized to cell nuclei, and its translocation was inhibited by the iNOS inhibitor 1400W. Immunohistological examination revealed that MMP-13 and Cbfa-1 expression levels are both reduced in 17-day-old embryos of iNOS-deficient mice. Silencing of Cbfa-1 mRNA blocked MMP-13 expression without interfering with endogenous NO production, confirming its role in NO-induced MMP-13 expression by MC3T3-E1 cells. The results described here suggest a mechanism by which NO regulates osteogenesis.es_ES
dc.language.isoenges_ES
dc.publisherThe Company of Biologistses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subject.meshGene Expression Regulation, Enzymologic es_ES
dc.subject.mesh3T3 Cells es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshCell Differentiation es_ES
dc.subject.meshCore Binding Factor Alpha 1 Subunit es_ES
dc.subject.meshCyclic GMP es_ES
dc.subject.meshCyclic GMP-Dependent Protein Kinases es_ES
dc.subject.meshEmbryo, Mammalianes_ES
dc.subject.meshGene Silencing es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMatrix Metalloproteinase 13 es_ES
dc.subject.meshMice es_ES
dc.subject.meshMice, Inbred C57BL es_ES
dc.subject.meshMice, Knockout es_ES
dc.subject.meshNitric Oxide es_ES
dc.subject.meshNitric Oxide Synthase Type II es_ES
dc.subject.meshOsteoblasts es_ES
dc.subject.meshOsteogenesis es_ES
dc.subject.meshRNA, Messenger es_ES
dc.subject.meshSecond Messenger Systems es_ES
dc.subject.meshTranscription, Genetices_ES
dc.titleCbfa-1 mediates nitric oxide regulation of MMP-13 in osteoblasts.es_ES
dc.typeArtículoes_ES
dc.identifier.pubmedID16636074es_ES
dc.format.volume119es_ES
dc.format.numberPt 9es_ES
dc.format.page1896-902es_ES
dc.identifier.doi10.1242/jcs.02895es_ES
dc.contributor.funderMinisterio de Ciencia y Tecnología (España)es_ES
dc.contributor.funderMinisterio de Educación y Ciencia (España)es_ES
dc.contributor.funderEuropean Regional Development Fund (ERDF/FEDER)es_ES
dc.contributor.funderComunidad de Madrides_ES
dc.contributor.funderSociedad Española de Nefrologíaes_ES
dc.contributor.funderRed Temática de Investigación Cooperativa en Enfermedades Cardiovasculares (España)es_ES
dc.contributor.funderMinisterio de Sanidad y Consumo (España)es_ES
dc.description.peerreviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1242/jcs.02895es_ES
dc.identifier.journalJournal of cell sciencees_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Antiguos CNICes_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2002-00399es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2005- 06025es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/2FD97-1432es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF 2000-0149es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2003- 01039es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/C03/01es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/embargoedAccesses_ES


Files in this item

Acceso Abierto
Thumbnail

This item appears in the following Collection(s)

Show simple item record