Post-lymphadenectomy Analysis of Exosomes from Lymphatic Exudate/Exudative Seroma of Melanoma Patients

Abstract

Circulating extracellular vesicles in biofluids became an interesting approach to analyse disease biomarkers. There are multiple methods for isolation of extracellular vesicles, differential ultracentrifugation is still considered as the gold-standard isolation technique for exosomes. Furthermore, exosomes purified by this method have demonstrated to display functional activity in vitro and in vivo and great versatility for subsequent analysis including electron microscopy, mass spectrometry or nucleic acid analysis. Here, we describe the method for isolation of exosomes from lymphatic exudate (seroma) obtained post-lymphadenectomy for liquid biopsy approaches