Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/11505
Título
Post-lymphadenectomy Analysis of Exosomes from Lymphatic Exudate/Exudative Seroma of Melanoma Patients
Autor(es)
Fecha de publicación
2020
Cita
En: Kristian M. Hargadon (ed.), Melanoma: Methods and Protocols, Methods in Molecular Biology, vol. 226.
Serie
Methods in Molecular Biology
Idioma
Inglés
Tipo de documento
book part
Resumen
Circulating extracellular vesicles in biofluids became an interesting approach to analyse
disease biomarkers. There are multiple methods for isolation of extracellular vesicles,
differential ultracentrifugation is still considered as the gold-standard isolation technique
for exosomes. Furthermore, exosomes purified by this method have demonstrated to
display functional activity in vitro and in vivo and great versatility for subsequent analysis
including electron microscopy, mass spectrometry or nucleic acid analysis. Here, we
describe the method for isolation of exosomes from lymphatic exudate (seroma) obtained
post-lymphadenectomy for liquid biopsy approaches
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