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dc.contributor.author | Mortuza, Gulnahar B | |
dc.contributor.author | Cavazza, Tommaso | |
dc.contributor.author | Garcia-Mayoral, Maria Flor | |
dc.contributor.author | Hermida, Dario | |
dc.contributor.author | Peset, Isabel | |
dc.contributor.author | Pedrero, Juan G | |
dc.contributor.author | Merino, Nekane | |
dc.contributor.author | Blanco, Francisco J | |
dc.contributor.author | Lyngsø, Jeppe | |
dc.contributor.author | Bruix, Marta | |
dc.contributor.author | Pedersen, Jan Skov | |
dc.contributor.author | Vernos, Isabelle | |
dc.contributor.author | Montoya, Guillermo | |
dc.date.accessioned | 2020-11-24T10:10:01Z | |
dc.date.available | 2020-11-24T10:10:01Z | |
dc.date.issued | 2014-09-29 | |
dc.identifier.citation | Nat Commun . 2014;5:5072. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/11403 | |
dc.description.abstract | chTOG is a conserved microtubule polymerase that catalyses the addition of tubulin dimers to promote microtubule growth. chTOG interacts with TACC3, a member of the transforming acidic coiled-coil (TACC) family. Here we analyse their association using the Xenopus homologues, XTACC3 (TACC3) and XMAP215 (chTOG), dissecting the mechanism by which their interaction promotes microtubule elongation during spindle assembly. Using SAXS, we show that the TACC domain (TD) is an elongated structure that mediates the interaction with the C terminus of XMAP215. Our data suggest that one TD and two XMAP215 molecules associate to form a four-helix coiled-coil complex. A hybrid methods approach was used to define the precise regions of the TACC heptad repeat and the XMAP215 C terminus required for assembly and functioning of the complex. We show that XTACC3 can induce the recruitment of larger amounts of XMAP215 by increasing its local concentration, thereby promoting efficient microtubule elongation during mitosis. | es_ES |
dc.description.sponsorship | This work was supported by the Ministerio de Economia y Competitividad (BFU2011-23815/BMC and Consolider CSD2006-00023 to G.M., M.B. and I.V.; CTQ2011-22514 to M.B.); the Fundacion Ramon Areces and the Comunidad Autonoma de Madrid (CAMS-2010/BMD-2305 to G.M. and M.B.). G.B.M. is supported by a RyC grant RyC-2008-03366. T.C. is supported by a FPI fellowship BES-2010-031355. J.S.P. is supported by The Danish Research Council for Independent Research Natural Sciences. F.J.B. is supported by the MINECO CTQ2011-28680 grant. We would also like to thank Andrian Valazquez for his advice and usage of ITC200; Jaminka Boskovic for helping with the EM grids; Marco Marenchino for advice and assistance with the Biacore; and Blanca Lopez for helpful tips on MALS experiments. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject.mesh | Amino Acid Sequence | es_ES |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Calorimetry | es_ES |
dc.subject.mesh | Circular Dichroism | es_ES |
dc.subject.mesh | Hydrogen-Ion Concentration | es_ES |
dc.subject.mesh | Magnetic Resonance Spectroscopy | es_ES |
dc.subject.mesh | Microtubule-Associated Proteins | es_ES |
dc.subject.mesh | Microtubules | es_ES |
dc.subject.mesh | Molecular Sequence Data | es_ES |
dc.subject.mesh | Mutation | es_ES |
dc.subject.mesh | Protein Structure, Tertiary | es_ES |
dc.subject.mesh | Scattering, Small Angle | es_ES |
dc.subject.mesh | Spindle Apparatus | es_ES |
dc.subject.mesh | Surface Plasmon Resonance | es_ES |
dc.subject.mesh | Temperature | es_ES |
dc.subject.mesh | Transcription Factors | es_ES |
dc.subject.mesh | Xenopus | es_ES |
dc.subject.mesh | Xenopus Proteins | es_ES |
dc.title | XTACC3-XMAP215 association reveals an asymmetric interaction promoting microtubule elongation. | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución-NoComercial-CompartirIgual 4.0 Internacional | * |
dc.identifier.pubmedID | 25262927 | es_ES |
dc.format.volume | 5 | es_ES |
dc.format.page | 5072 | es_ES |
dc.identifier.doi | 10.1038/ncomms6072 | es_ES |
dc.contributor.funder | Comunidad de Madrid (España) | |
dc.contributor.funder | Fundación Ramón Areces | |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | ICREA | |
dc.contributor.funder | Danish Research Council | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2041-1723 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1038/ncomms6072 | es_ES |
dc.identifier.journal | Nature communications | es_ES |
dc.repisalud.institucion | CNIO | es_ES |
dc.repisalud.orgCNIO | CNIO::Grupos de investigación::Antiguos CNIO | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/BFU2011-23815/BMC | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/CSD2006-00023 | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/CTQ2011-22514 | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/BES-2010-031355 | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/RyC-2008-03366 | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/CAMS-2010/BMD-2305 | es_ES |
dc.relation.projectID | info:eu_repo/grantAgreement/ES/CTQ2011-28680 | es_ES |
dc.rights.accessRights | open access | es_ES |