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dc.contributor.authorMortuza, Gulnahar B
dc.contributor.authorCavazza, Tommaso
dc.contributor.authorGarcia-Mayoral, Maria Flor
dc.contributor.authorHermida, Dario
dc.contributor.authorPeset, Isabel
dc.contributor.authorPedrero, Juan G
dc.contributor.authorMerino, Nekane
dc.contributor.authorBlanco, Francisco J
dc.contributor.authorLyngsø, Jeppe
dc.contributor.authorBruix, Marta
dc.contributor.authorPedersen, Jan Skov
dc.contributor.authorVernos, Isabelle
dc.contributor.authorMontoya, Guillermo 
dc.date.accessioned2020-11-24T10:10:01Z
dc.date.available2020-11-24T10:10:01Z
dc.date.issued2014-09-29
dc.identifier.citationNat Commun . 2014;5:5072.es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/11403
dc.description.abstractchTOG is a conserved microtubule polymerase that catalyses the addition of tubulin dimers to promote microtubule growth. chTOG interacts with TACC3, a member of the transforming acidic coiled-coil (TACC) family. Here we analyse their association using the Xenopus homologues, XTACC3 (TACC3) and XMAP215 (chTOG), dissecting the mechanism by which their interaction promotes microtubule elongation during spindle assembly. Using SAXS, we show that the TACC domain (TD) is an elongated structure that mediates the interaction with the C terminus of XMAP215. Our data suggest that one TD and two XMAP215 molecules associate to form a four-helix coiled-coil complex. A hybrid methods approach was used to define the precise regions of the TACC heptad repeat and the XMAP215 C terminus required for assembly and functioning of the complex. We show that XTACC3 can induce the recruitment of larger amounts of XMAP215 by increasing its local concentration, thereby promoting efficient microtubule elongation during mitosis.es_ES
dc.description.sponsorshipThis work was supported by the Ministerio de Economia y Competitividad (BFU2011-23815/BMC and Consolider CSD2006-00023 to G.M., M.B. and I.V.; CTQ2011-22514 to M.B.); the Fundacion Ramon Areces and the Comunidad Autonoma de Madrid (CAMS-2010/BMD-2305 to G.M. and M.B.). G.B.M. is supported by a RyC grant RyC-2008-03366. T.C. is supported by a FPI fellowship BES-2010-031355. J.S.P. is supported by The Danish Research Council for Independent Research Natural Sciences. F.J.B. is supported by the MINECO CTQ2011-28680 grant. We would also like to thank Andrian Valazquez for his advice and usage of ITC200; Jaminka Boskovic for helping with the EM grids; Marco Marenchino for advice and assistance with the Biacore; and Blanca Lopez for helpful tips on MALS experiments.es_ES
dc.language.isoenges_ES
dc.publisherNature Publishing Group es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAmino Acid Sequence es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshCalorimetry es_ES
dc.subject.meshCircular Dichroism es_ES
dc.subject.meshHydrogen-Ion Concentration es_ES
dc.subject.meshMagnetic Resonance Spectroscopy es_ES
dc.subject.meshMicrotubule-Associated Proteins es_ES
dc.subject.meshMicrotubules es_ES
dc.subject.meshMolecular Sequence Data es_ES
dc.subject.meshMutation es_ES
dc.subject.meshProtein Structure, Tertiary es_ES
dc.subject.meshScattering, Small Angle es_ES
dc.subject.meshSpindle Apparatus es_ES
dc.subject.meshSurface Plasmon Resonance es_ES
dc.subject.meshTemperature es_ES
dc.subject.meshTranscription Factors es_ES
dc.subject.meshXenopus es_ES
dc.subject.meshXenopus Proteins es_ES
dc.titleXTACC3-XMAP215 association reveals an asymmetric interaction promoting microtubule elongation.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID25262927es_ES
dc.format.volume5es_ES
dc.format.page5072es_ES
dc.identifier.doi10.1038/ncomms6072es_ES
dc.contributor.funderComunidad de Madrid (España) 
dc.contributor.funderFundación Ramón Areces 
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.contributor.funderICREA
dc.contributor.funderDanish Research Council
dc.description.peerreviewedes_ES
dc.identifier.e-issn2041-1723es_ES
dc.relation.publisherversionhttps://doi.org/10.1038/ncomms6072es_ES
dc.identifier.journalNature communicationses_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Antiguos CNIOes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/BFU2011-23815/BMCes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/CSD2006-00023es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/CTQ2011-22514es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/BES-2010-031355es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/RyC-2008-03366es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/CAMS-2010/BMD-2305es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/CTQ2011-28680es_ES
dc.rights.accessRightsopen accesses_ES


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