Mostrar el registro sencillo del ítem
dc.contributor.author | Scarola, Michele | |
dc.contributor.author | Comisso, Elisa | |
dc.contributor.author | Pascolo, Rhena | |
dc.contributor.author | Chiaradia, Riccardo | |
dc.contributor.author | Marion, RM | |
dc.contributor.author | Schneider, Claudio | |
dc.contributor.author | Blasco, MA | |
dc.contributor.author | Schoeftner, Stefan | |
dc.contributor.author | Benetti, Roberta | |
dc.date.accessioned | 2020-05-08T17:30:56Z | |
dc.date.available | 2020-05-08T17:30:56Z | |
dc.date.issued | 2015-07-09 | |
dc.identifier.citation | Nat Commun. 2015;6:763 | es_ES |
dc.identifier.issn | 2041-1723 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/10007 | |
dc.description.abstract | Pseudogene-derived, long non-coding RNAs (lncRNAs) act as epigenetic regulators of gene expression. Here we present a panel of new mouse Oct4 pseudogenes and demonstrate that the X-linked Oct4 pseudogene Oct4P4 critically impacts mouse embryonic stem cells (mESCs) self-renewal. Sense Oct4P4 transcription produces a spliced, nuclear-restricted lncRNA that is efficiently upregulated during mESC differentiation. Oct4P4 lncRNA forms a complex with the SUV39H1 HMTase to direct the imposition of H3K9me3 and HP1α to the promoter of the ancestral Oct4 gene, located on chromosome 17, leading to gene silencing and reduced mESC self-renewal. Targeting Oct4P4 expression in primary mouse embryonic fibroblasts causes the re-acquisition of self-renewing features of mESC. We demonstrate that Oct4P4 lncRNA plays an important role in inducing and maintaining silencing of the ancestral Oct4 gene in differentiating mESCs. Our data introduces a sense pseudogene-lncRNA-based mechanism of epigenetic gene regulation that controls the cross-talk between pseudogenes and their ancestral genes. | es_ES |
dc.description.sponsorship | We thank Alessandro Marcello (ICGEB Trieste, Italy) for providing the basic vectors for the MS2 tethering model. M.S. is supported by a AIRC post-doctoral fellowship; E.C. is enroled in the PhD program for Molecular Medicine at the University of Trieste. This work was supported by an Italian Association for Cancer Research (AIRC) grant Rif 42/08, 6352 to R.B., an Italian Association for Cancer Research (AIRC) grant cod. 10299 and a Fondazione Veronesi grant to S.S. and by a grant from the Italian Ministry of Education, Universities and Research (MIUR) CTN01_00177_817708 to C.S. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Cell Line | es_ES |
dc.subject.mesh | Cell Line, Tumor | es_ES |
dc.subject.mesh | Cell Self Renewal | es_ES |
dc.subject.mesh | Chromatin Immunoprecipitation | es_ES |
dc.subject.mesh | Chromosomal Proteins, Non-Histone | es_ES |
dc.subject.mesh | Epigenesis, Genetic | es_ES |
dc.subject.mesh | Histones | es_ES |
dc.subject.mesh | Immunoprecipitation | es_ES |
dc.subject.mesh | Methyltransferases | es_ES |
dc.subject.mesh | Mice | es_ES |
dc.subject.mesh | Mouse Embryonic Stem Cells | es_ES |
dc.subject.mesh | NIH 3T3 Cells | es_ES |
dc.subject.mesh | Octamer Transcription Factor-3 | es_ES |
dc.subject.mesh | Pseudogenes | es_ES |
dc.subject.mesh | RNA, Long Noncoding | es_ES |
dc.subject.mesh | Repressor Proteins | es_ES |
dc.subject.mesh | Gene Expression Regulation, Developmental | es_ES |
dc.title | Epigenetic silencing of Oct4 by a complex containing SUV39H1 and Oct4 pseudogene lncRNA | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución-NoComercial-CompartirIgual 4.0 Internacional | * |
dc.identifier.pubmedID | 26158551 | es_ES |
dc.format.volume | 6 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 7631 | es_ES |
dc.identifier.doi | 10.1038/ncomms8631 | es_ES |
dc.contributor.funder | Italian Association for Cancer Research | |
dc.contributor.funder | Fondazione Umberto Veronesi | |
dc.contributor.funder | Ministero dell Istruzione, dell Universita e della Ricerca (Italia) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2041-1723 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1038/ncomms8631. | es_ES |
dc.identifier.journal | Nature communications | es_ES |
dc.repisalud.institucion | CNIO | es_ES |
dc.repisalud.orgCNIO | CNIO::Grupos de investigación::Grupo de Telómeros y Telomerasa | es_ES |
dc.rights.accessRights | open access | es_ES |